The effect of different alleles at the r locus on the synthesis of seed storage proteins in Pisum sativum

Plant Molecular Biology
S R TurnerR Casey

Abstract

Rocket immunoelectrophoresis was used to measure the accumulation of storage proteins in developing cotyledons of two Pisum sativum (pea) genotypes, that were close to isogenic except for the nature of the allele at the r locus. There was a marked decrease in legumin accumulation in the rr (wrinkled-seeded) genotype compared to the RR (round-seeded) genotype. The accumulation of vicilin did not differ greatly between the two genotypes. Pulse-labelling studies indicated that the differences in rates of accumulation of legumin between the rr and RR genotypes were a consequence of differences in rates of protein synthesis. Measurements of relative amounts of specific mRNAs, using cDNA clones as probes, showed lower amounts of legumin mRNA in developing cotyledons of the rr, compared to the RR, genotype. Both vicilin mRNAs and convicilin mRNA, the latter of which shows a similar temporal pattern of expression to those of the major legumin species, are relatively unaffected by the nature of the allele at the r locus. Nuclear run-on transcription experiments indicated no differences in the rate of synthesis of legumin transcripts in the rr and RR near-isolines. The consequences of homozygosity for the r allele on storage protein mRNA...Continue Reading

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Citations

Jun 1, 1996·Plant Molecular Biology·V K Plotnikov, N B Bakaldina
Jul 1, 1995·The Plant Cell·C Martin, A M Smith
Oct 18, 2008·Comptes rendus biologies·Karine GallardoJudith Burstin
Sep 1, 1992·The New Phytologist·Alison M Smith, Kay Denyer
Jan 19, 2006·Journal of Agricultural and Food Chemistry·Emmanouil N TzitzikasRichard G F Visser

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