PMID: 8952944Apr 1, 1996Paper

The effect of different levels of the B800-850 light-harvesting complex on intracytoplasmic membrane development in Rhodobacter sphaeroides

Archives of Microbiology
James N Sturgis, R A Niedermann

Abstract

A role for the peripheral (B800-850) light-harvesting complex in vesicularization of the Rhodobacter sphaeroides intracytoplasmic membrane (ICM), suggested from studies in mutant strains lacking one or more of the pigment-protein complexes, was examined further in the wild-type strain NCIB 8253 grown at high ( approximately 1000 W m-2), moderate ( approximately 300 W m-2), and low ( approximately 100 W m-2) light intensities. The resulting ICM vesicles (chromatophores) had B800-850 levels related inversely to irradiance and banded in rate-zone sedimentation at approximately 1.10, 1.09, and 1.07 g ml-1, respectively. Equilibrium centrifugation on iso-osmotic gradients indicated that this distinct sedimentation behavior resulted solely from differences in hydrodynamic radii. These size differences were confirmed by gel-exclusion chromatography and in electron micrographs of thin-sectioned cells. A pulse-chase study of ICM growth following a tenfold reduction in light intensity showed a relatively slow equilibration of membrane proteins during adaptation, and that new protein was incorporated largely into additional ICM formed at the lowered illumination level, giving rise to chromatophores of reduced size and elevated B800-850 co...Continue Reading

Citations

Oct 26, 2013·European Biophysics Journal : EBJ·Jean-Pierre Duneau, James N Sturgis
Aug 27, 2004·Nature·Svetlana BahatyrovaC Neil Hunter
Dec 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Raoul N FreseRienk van Grondelle
Aug 28, 2010·Journal of Structural Biology·Lu-Ning LiuSimon Scheuring
Sep 11, 2007·Biophysical Journal·Raoul N FreseRienk van Grondelle
Jun 5, 2012·Biochimica Et Biophysica Acta·Peter G Adams, C Neil Hunter
Apr 2, 2014·Biochimica Et Biophysica Acta·Simon ScheuringZiv Reich
Aug 27, 2016·ELife·Melih SenerKlaus Schulten
Oct 27, 2007·Biochimica Et Biophysica Acta·Francesca GubelliniB Andrea Melandri
Feb 26, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jen HsinKlaus Schulten
Apr 30, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ying ShiJian-Ping Zhang
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·P LundahlE Greijer
Mar 7, 2009·Biochemistry·James N SturgisRobert A Niederman
Jul 16, 2005·Science·Simon Scheuring, James N Sturgis

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