The effect of diluting povidone-iodine on bacterial growth associated with speech

BMC Ophthalmology
Sivashanth GnanasekaranSukhpal S Sandhu

Abstract

Povidone-Iodine (PI) may be diluted when used as an antiseptic prior to an intravitreal injection in an attempt to decrease patient discomfort. This study aims to investigate the effect of diluting povidone-iodine (PI) on bacterial growth from bacterial droplet dispersal associated with speech. Participants read a standardised script for 5 min over a blood agar plate positioned at 20 cm in a simulated position of an intravitreal injection procedure. The blood agar plates were subject to a randomised pre-application of 1% PI; 2.5% PI; 5% PI and no pre-application (control). The plates were incubated at 37 °C for 72 h and the number of Colony Forming Units (CFUs) was determined. CFUs were summarised as median and interquartile range (IQR). Wilcoxon rank sum test was used to assess pairwise comparisons of the various PI concentrations to the control group. Any trend across PI concentration was assessed using Kendall's tau rank correlation. Twenty-one subjects participated. Control plates had a median growth of 25 CFUs (interquartile range [IQR]:15-40), 1% PI plates had a median growth of 30 CFUs (IQR:15-82), 2.5% PI had a median growth of 18 CFUs (IQR:10-32) and 5% PI had a median growth of 2 CFUs (IQR:0-5). There was significantl...Continue Reading

References

Dec 1, 1991·Ophthalmology·M G Speaker, J A Menikoff
Apr 1, 1982·Journal of Clinical Microbiology·R L BerkelmanR L Anderson
Nov 1, 1981·Journal of Clinical Microbiology·G E Buck, M T Kelly
May 1, 1996·Cornea·W D MathersM B Zimmerman
Jul 13, 2006·Cutaneous and Ocular Toxicology·Karen E Lerhaupt, Thomas F Mauger
Oct 6, 2006·The New England Journal of Medicine·Philip J RosenfeldUNKNOWN MARINA Study Group
Oct 6, 2006·The New England Journal of Medicine·David M BrownUNKNOWN ANCHOR Study Group
Dec 2, 2008·Environmental Microbiology·Marcus C de GoffauHermie J M Harmsen
Aug 22, 2009·Cutaneous and Ocular Toxicology·Jin JiangTing Shen
Dec 21, 2010·American Journal of Ophthalmology·Amy E Green-SimmsSophie J Bakri
May 17, 2011·Investigative Ophthalmology & Visual Science·Qunfeng DongValery I Shestopalov
Aug 10, 2011·Archives of Ophthalmology·Joanne C WenOmai B Garner
Oct 23, 2012·Ophthalmology·Jeffrey S HeierUNKNOWN VIEW 1 and VIEW 2 Study Groups
Jan 21, 2015·Ophthalmology·Travis A MeredithUNKNOWN Comparison of Age-Related Macular Degeneration Treatments Trials Research Group
Feb 19, 2015·The New England Journal of Medicine·UNKNOWN Diabetic Retinopathy Clinical Research NetworkRoy W Beck
Feb 26, 2015·Arquivos brasileiros de oftalmologia·Helio F ShiromaEduardo Buchele Rodrigues
Jul 23, 2015·Retina·Sunir J GargUNKNOWN Post-Injection Endophthalmitis Study Team
Jul 28, 2016·American Journal of Ophthalmology·Bobeck S ModjtahediDean Eliott

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Citations

Jan 19, 2020·BMC Ophthalmology·Jagger Koerner, Andrzej Grzybowski
Jan 27, 2020·International Ophthalmology·Alfonso Vasquez-PerezFrancesco Aiello
Jan 11, 2020·Survey of Ophthalmology·Shriji N PatelStephen J Kim
Nov 2, 2021·Current Opinion in Ophthalmology·Andrzej GrzybowskiJagger Koerner

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