PMID: 108168Jan 1, 1978

The effect of divalent cations on aggregation of Dictyostelium discoideum

Differentiation; Research in Biological Diversity
W F LoomisP Brachet


Following the initiation of development, amoebae of Dictyostelium discoideum aggregate chemotactically toward cyclic AMP (cAMP). Adenyl cyclase, cAMP phosphodiesterase, and cAMP binding sites all increase 20--40 fold during the first few hours of development. It has been shown that addition of 1 mM EDTA and 5 mM MgCl2 accelerates the aggregation process. Likewise, the calcium ionophore, A23187, leads to precocious aggregation while 4 X 10(-5) M progesterone considerably delays it. These treatments have now been shown to result in increased accumulation of adenyl cyclase in the case of EDTA and Mg2+ or the ionophore and greatly decreased accumulation in the case of the steroid. Treatment with EDTA and Mg2+ or the ionophore has been shown not only to accelerate aggregation in wild-type amoebae but to overcome complete blocks to aggregation in certain mutant strains. We have found that addition of Mn2+ will also permit aggregation of mutant cells otherwise unable to aggregate. This divalent ion, unlike EDTA and Mg2+ or the ionophore, was shown to directly stimulate adenyl cyclase. Calcium ions were also found to affect the enzyme such that at Ca2+ concentrations found within the cells the great majority of the activity is inhibite...Continue Reading


Jul 8, 1975·Biochemical and Biophysical Research Communications·G Gerisch, U Wick
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M DarmonL H Da Silva
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A Goldbeter, L A Segel
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May 1, 1979·Developmental Biology·W F Loomis
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·P M Janssens
Jun 1, 1991·Research in Microbiology·T WincklerR Mutzel
Dec 8, 1998·Molecular Biology of the Cell·M T Laub, W F Loomis
Mar 4, 1998·Cell Motility and the Cytoskeleton·E LeeD A Knecht
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Jan 23, 2016·Development Genes and Evolution·Johanna Márquez LópezSascha Thewes

Related Concepts

Adenylate Cyclase
Cations, Divalent
Cell Aggregation
Dictyostelium discoideum
Edetic Acid, Sodium Salt
Progesterone, (9 beta,10 alpha)-Isomer

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