PMID: 108168Jan 1, 1978

The effect of divalent cations on aggregation of Dictyostelium discoideum

Differentiation; Research in Biological Diversity
W F LoomisP Brachet

Abstract

Following the initiation of development, amoebae of Dictyostelium discoideum aggregate chemotactically toward cyclic AMP (cAMP). Adenyl cyclase, cAMP phosphodiesterase, and cAMP binding sites all increase 20--40 fold during the first few hours of development. It has been shown that addition of 1 mM EDTA and 5 mM MgCl2 accelerates the aggregation process. Likewise, the calcium ionophore, A23187, leads to precocious aggregation while 4 X 10(-5) M progesterone considerably delays it. These treatments have now been shown to result in increased accumulation of adenyl cyclase in the case of EDTA and Mg2+ or the ionophore and greatly decreased accumulation in the case of the steroid. Treatment with EDTA and Mg2+ or the ionophore has been shown not only to accelerate aggregation in wild-type amoebae but to overcome complete blocks to aggregation in certain mutant strains. We have found that addition of Mn2+ will also permit aggregation of mutant cells otherwise unable to aggregate. This divalent ion, unlike EDTA and Mg2+ or the ionophore, was shown to directly stimulate adenyl cyclase. Calcium ions were also found to affect the enzyme such that at Ca2+ concentrations found within the cells the great majority of the activity is inhibite...Continue Reading

References

Jul 8, 1975·Biochemical and Biophysical Research Communications·G Gerisch, U Wick
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M DarmonL H Da Silva
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A Goldbeter, L A Segel
Oct 1, 1978·Developmental Biology·J F Grutsch, A Robertson
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell
Sep 1, 1970·The Biochemical Journal·D J Watts, J M Ashworth

Citations

May 1, 1979·Developmental Biology·W F Loomis
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·P M Janssens
Jun 1, 1991·Research in Microbiology·T WincklerR Mutzel
Dec 8, 1998·Molecular Biology of the Cell·M T Laub, W F Loomis
Mar 4, 1998·Cell Motility and the Cytoskeleton·E LeeD A Knecht
Jan 1, 1991·Developmental Genetics·D R FontanaJ C Phillips
Jan 1, 1984·FEMS Microbiology Letters·G N Europe-FinnerP C Newell
Jan 1, 1982·Biophysics of Structure and Mechanism·D MalchowU Gras
Jan 23, 2016·Development Genes and Evolution·Johanna Márquez LópezSascha Thewes

Related Concepts

Calcimycin
Adenylate Cyclase
Calcium
Cations, Divalent
Cell Aggregation
Dictyostelium discoideum
Edetic Acid, Sodium Salt
Magnesium
Manganese
Progesterone, (9 beta,10 alpha)-Isomer

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