The effect of endotoxins and enzymes in vitro on the release of gingival histamine

Journal of Periodontology
J Schwartz, M Dibblee

Abstract

Gingival samples removed from fifteen Beagle dogs were sectioned into small pieces, parts of which served as the uncultured piece; the remaining pieces were organ cultured for four hours at 37 degrees C in MEM control, compound 48/80, endotoxins, protease, collagenase, hyaluronidase, trypsin and chymotrypin media. Uncultured and cultured tissues and spent media were analyzed spectrofluorometrically for histamine content. The uncultured gingiva contained a mean of 2.80 mug histamine/g of tissue and was considered to contain 100% total histamine available for release. The percentages of histamine released into the medium were 5.4% for culture control, 57.3% for compound 48/80, 5.4% for endotoxins, 77.3% for protease, 16.1% for hyaluronidase, 24 for collagenase, 39.3% for trypsin, and 36.5% for chymotrypsin. When compared to the culture control, all test substances showed statistically significant histamine release (P less than 0.005 to P less than 0.0005) except for the endotoxins and for hyaluronidase (P greater than 0.05). The results demonstrate (1) that gingiva contains a potential source or reservoir of histamine, presumably in mast cells, and when appropriately challenged in vitro can release this histamine; (2) no direct e...Continue Reading

References

Mar 1, 1975·Journal of Periodontology·J Schwartz, M Dibblee
Jan 1, 1966·Journal of Periodontal Research·E TheiladeH Löe
Sep 1, 1969·Journal of Dental Research·H M FullmerK A Whedon
Mar 1, 1970·Journal of Dental Research·S S Socransky
May 1, 1972·Journal of Periodontology·J SchwartzR B Parker
Aug 1, 1973·Archives of Oral Biology·R R Ranney, E H Montgomery
Jan 1, 1969·Archives of Oral Biology·S E BerglundS E Mergenhagen
Jul 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·G ASBOE-HANSEN, D GLICK
Feb 1, 1959·The American Journal of Physiology·R W SCHAYERP BIZONY
Apr 1, 1961·Journal of Bacteriology·R J GIBBONS, J B MACDONALD
Oct 1, 1970·Infection and Immunity·W A HookS E Mergenhagen

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Citations

Sep 1, 1986·Journal of Clinical Periodontology·O FardalE Freeman

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