The effect of energy balance on the transcriptome of bovine granulosa cells at 60 days postpartum

Theriogenology
Annie GirardMarc-André Sirard

Abstract

Dairy cows expend great amounts of energy during the lactation peak to cope with milk production. A state of negative energy balance (NEB) was suggested as a cause for the suboptimal fertility observed during this period, via an interaction with ovarian function. The objective of this study was to identify the impact of NEB on gene expression in granulosa cells of dairy cows at 60 days postpartum and to suggest a potential treatment to improve ovarian function. Dairy cows at 60 days postpartum from 10 typical medium-sized farms were synchronized using a single injection of prostaglandin. Dominant follicles  were collected 42 hours later by transvaginal aspiration. Blood concentrations of beta-hydroxybutyrate (BHB) on the day of aspiration were used to classify animals into two groups: severe NEB (high BHB, n = 12) and mild NEB (low BHB, n = 12). The transcriptomes of granulosa cells from both groups were contrasted using microarrays, and the differentially expressed genes were analyzed using Ingenuity Pathway Analysis to identify affected functions and potential upstream regulators. Genes linked with cellular organization (KRT4 and PPL), proliferation (TACSTD2), and fatty acids metabolism (VNN2) were downregulated in granulosa ...Continue Reading

References

Mar 1, 1988·Journal of Reproduction and Fertility·F J Schweigert, H Zucker
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H KellerW Wahli
Jul 20, 1999·Molecular and Cellular Endocrinology·A E Drummond, J K Findlay
Dec 11, 1999·Cells, Tissues, Organs·J RohwedelA M Wobus
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
May 16, 2002·Histopathology·P G Chu, L M Weiss
Nov 6, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·Alexandra L L DurlingerAxel P N Themmen
Mar 5, 2004·Animal Reproduction Science·H LopezM C Wiltbank
Mar 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·C KendziorskiM N Gould
Nov 1, 2005·Theriogenology·Marc-André SirardClaude Robert
Jan 20, 2006·Reproduction in Domestic Animals = Zuchthygiene·T VanholderG Opsomer
Mar 24, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Catherine Gallou-KabaniClaudine Junien
Apr 22, 2008·International Journal of Cancer. Journal International Du Cancer·Carlo Stresemann, Frank Lyko
Dec 5, 2008·Nutrition Reviews·Sam Samuel, Michael D Sitrin
Jan 28, 2009·Nature Reviews. Immunology·J Rodrigo MoraUlrich H von Andrian
Mar 12, 2010·Nutrition Journal·Cynthia A DaleyStephanie Larson
Apr 24, 2010·Journal of Dairy Science·L Hymøller, S K Jensen
Jul 16, 2010·The Journal of Reproduction and Development·Stephen LeBlanc
Jul 16, 2010·The Journal of Reproduction and Development·Stephen LeBlanc
Mar 25, 2011·The Journal of Clinical Endocrinology and Metabolism·Paul A FowlerPeter J O'Shaughnessy
Dec 14, 2011·Biochemical and Biophysical Research Communications·Belinda J KaskowLawrence J Abraham
Mar 30, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle GilbertMarc-André Sirard
May 30, 2013·Molecular Endocrinology·Lane K ChristensonJens Vanselow
Sep 6, 2013·Biology of Reproduction·Khampoun SayasithJacques G Lussier

❮ Previous
Next ❯

Citations

May 10, 2016·Theriogenology·Pat LonerganDimitrios Rizos
Apr 20, 2017·Reproduction, Fertility, and Development·David A LandryMarc-André Sirard
Nov 28, 2017·Journal of Dairy Science·S J HolcombeL M Sordillo
May 20, 2020·Biology of Reproduction·Patricia G Tremblay, Marc-André Sirard
Feb 13, 2020·Scientific Reports·Mikhael PoirierMichael Hoelker
Jul 27, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Ying ZhangMarc-André Sirard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.