PMID: 9447701Jul 1, 1997Paper

The effect of epidermal growth factor and transforming growth factor beta 1 on proliferation and differentiation of urothelial cells in urinary bladder explant culture

Biology of the Cell
M SterleU Batista

Abstract

The effects of two growth factors, EGF and TGF beta 1, on growth and differentiation of different populations of urothelial cells in explant cultures of mouse urinary bladder have been studied by electron microscopy and lectin analysis. In an explant culture 10 days after the implantation three different populations of urothelial cells can be distinguished. Original urothelial cells, which are integral part of the explant, new urothelial cells, which cover the baso-lateral sides of the explant and are organized in a multilayer epithelium, and new urothelial cells, which are not any more in direct contact with the explant and grow over the membrane in epithelium-like structure. Exogenously added EGF or TGF beta 1 did not affect either the formation or the thickness of multilayered urothelium, so cells were proliferating on the free surfaces of stroma as well as on the epithelium expanding over the membrane. In the absence of growth factors in medium, the newly formed baso-lateral multilayered epithelium bordering the stroma shows ultrastructural signs of terminal differentiation suggesting that for cell proliferation and differentiation the action of stroma is of crucial importance. On the other hand, the differentiation of the ...Continue Reading

References

Jun 1, 1976·Journal of Cellular Physiology·G Carpenter, S Cohen
May 1, 1975·Biological Reviews of the Cambridge Philosophical Society·R M Hicks
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A OberhammerR Schulte-Hermann
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J RotelloL E Gerschenson
May 1, 1989·Cell and Tissue Kinetics·F J Chlapowski
Jan 1, 1985·Journal of Cell Science. Supplement·G Carpenter
Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L J Loretz, C A Reznikoff
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D KirkL W Jones
Feb 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W I De BoerT H Van der Kwast
Nov 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A BenedettiA M Jezequel
Aug 4, 1994·Nature·J L WranaJ Massagué
Feb 1, 1965·The British Journal of Dermatology·I SARKANYG A CARON

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Citations

May 29, 2002·Urology·Brian J FlynnLawrence I Rothblum
Aug 12, 1998·Nature Structural Biology·E A Berger
Nov 11, 1998·Nature Medicine·D D Richman
May 9, 2009·Nature·Ananyo Bhattacharya
Feb 25, 2005·Nature·Bing ChenStephen C Harrison
Apr 18, 1998·Nature Structural Biology·R A FurutaC D Weiss
Oct 18, 2011·Nature Structural & Molecular Biology·Mikyung KimEllis L Reinherz
Oct 25, 2013·Nature Structural & Molecular Biology·Alberto BartesaghiSriram Subramaniam
Jul 25, 2006·Nature Structural & Molecular Biology·Micah A LuftigAndrea Carfí
Dec 14, 2004·Nature Structural & Molecular Biology·Aymeric de ParsevalJohn H Elder
Oct 20, 2000·In Vitro Cellular & Developmental Biology. Animal·W LiuL Baskin
Nov 25, 2014·Nature Structural & Molecular Biology·Gregory H BirdLoren D Walensky
Sep 29, 2006·The EMBO Journal·Sophia Rits-VollochBing Chen
Jun 23, 2015·Nature Structural & Molecular Biology·Young Do KwonPeter D Kwong
Apr 17, 2007·The Journal of Pharmacology and Experimental Therapeutics·Maurits M BarendrechtMartin C Michel

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