The effect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells

Advanced Biomedical Research
Farzaneh SadeghiFateme Shabani

Abstract

During adolescence, sex hormones play an important role in regulating proliferation, differentiation, maturation, and the scheduled death of chondrocytes. Although some studies have reported the regulatory role of estrogen in the development and progression of cartilage, some of the mechanisms still remain unclear, including the role of estrogen in the expression of cartilage-specific genes in chondrogenesis process, which we cover in this study. In the present study, we used adipose-derived stem cells (ADSCs) to differentiate into cartilage. Differentiated cartilage cells were used in the control (without estrogen E2 in the culture medium) and experimental (with estrogen in the culture medium) groups to evaluate the expression of type II collagen and aggrecan as chondrogenic genes markers, with -real-time polymerase chain reaction technique. Our results indicated that estrogen leads to inhibition of type II collagen gene expression and reduction of aggrecan gene expression. Therefore, estrogen probably has negative effects on chondrogenesis process of ADSCs.

References

Apr 1, 1984·The Journal of Clinical Endocrinology and Metabolism·F G CassorlaD L Loriaux

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Image J

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Related Papers

Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association
Hiroyuki TakanoEiji Itoi
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Haiwei XuLi Cui
© 2021 Meta ULC. All rights reserved