The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles.

Journal of Assisted Reproduction and Genetics
Martin WildingBrian Dale

Abstract

To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles. A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential. Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences. Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures.

References

Nov 1, 1976·Fertility and Sterility·D H MaasL Mastroianni
Apr 1, 1990·Journal of in Vitro Fertilization and Embryo Transfer : IVF·B FischY Tadir
Oct 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·D M SaundersJ Cohen
Mar 1, 1974·Journal of Reproduction and Fertility·R G Edwards
Jan 14, 1967·Nature·P Mitchell, J Moyle
Mar 1, 1972·Journal of Reproduction and Fertility·R ShalgiN Soferman
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Jan 1, 1995·Methods in Enzymology·M ReersL B Chen
Jun 10, 1998·Human Reproduction·B DaleM Wilding
Mar 31, 2000·Human Reproduction·K P PhillipsJ M Baltz
Apr 2, 2002·Human Reproduction·Carmen MendozaJan Tesarik
Jul 1, 1955·American Journal of Obstetrics and Gynecology·L G FEO

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Citations

Nov 2, 2011·Analytical Chemistry·Eva Baldrich, Francesc X Muñoz

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