The effect of fasting and refeeding on mRNA expression of PepT1 and gastrointestinal hormones regulating digestion and food intake in zebrafish (Danio rerio).

Fish Physiology and Biochemistry
William Koven, Patricia Schulte

Abstract

In vertebrates, a significant part of ingested protein is absorbed as di- and tripeptides through a brush border membrane proton/oligopeptide transporter protein called PepT1. The aim of the present study was to determine the effect of short-term food deprivation and refeeding in adult zebrafish (Danio rerio) on gastrointestinal mRNA expression of PepT1 as well as on the satiety hormones cholecystokinin (CCK), gastrin-releasing peptide (GRP) and ghrelin (GHR) in order to elucidate a potential mechanism driving compensatory growth. Sixty adult zebrafish were stocked in a 40-L aquarium and fed daily a commercial flake diet to satiation for 10 days where the digestive tracts (DT) of sampled fish (n = 5) were dissected out. Samplings were repeated following 1, 2 and 5 days of food deprivation and after 1, 2 and 5 days of refeeding. The RNA was extracted from all sampled DTs and analyzed by quantitative real-time PCR for the mRNA expression of PepT1, rRNA 18S, CCK, GRP and GHR. PepT1 mRNA expression increased with successive refeedings reaching a level approximately 8 times higher than pre-fast levels. CCK, GRP and GHR mRNA levels also decreased during fasting, but increased only to pre-fasting levels with refeeding. Overall, the re...Continue Reading

References

Nov 1, 1990·The American Journal of Physiology·S Fiorucci, K E McArthur
Jan 11, 1990·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M C ThorndykeS R Vigna
Aug 1, 1985·The American Journal of Physiology· Ganapathy, F H Leibach
Apr 20, 2000·European Journal of Pharmacology·A MuraiG J Dockray
Aug 15, 2000·Annual Review of Nutrition·R P Ferraris, H V Carey
Jan 18, 2002·Endocrinology·Michela BagnascoSatya P Kalra
Oct 25, 2002·Pflügers Archiv : European journal of physiology·D J KennedyD T Thwaites
Mar 28, 2003·American Journal of Physiology. Cell Physiology·Alex W CohenMichael P Lisanti
Oct 17, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Siamak A Adibi
Feb 24, 2004·Annual Review of Physiology·Hannelore Daniel
Mar 5, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Luis Fabián CanosaRichard Ector Peter
Mar 8, 2005·General and Comparative Endocrinology·Saad ShoushaNoboru Murakami
Jun 3, 2005·The Journal of Nutrition·N P DarcelH E Raybould
Jul 28, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ashild Krogdahl, Anne Marie Bakke-McKellep
Sep 27, 2005·Biochemical and Biophysical Research Communications·Kimio MatsumuraSusumu Seino
Apr 25, 2006·General and Comparative Endocrinology·Elisabeth JönssonBjörn Thrandur Björnsson
May 12, 2006·Peptides·Kouhei MatsudaSeiji Shioda
Jun 9, 2006·Journal of Medicinal Chemistry·Balvinder S VigTeresa N Faria
Jan 24, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brian S ShepherdGregory M Weber
May 1, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Elisabeth JönssonBjörn Thrandur Björnsson
Jun 29, 2007·General and Comparative Endocrinology·Genciana TerovaMarco Saroglia
Jul 20, 2007·Pflügers Archiv : European journal of physiology·Caroline HaboldJean-Hervé Lignot
Nov 6, 2007·The Journal of Experimental Biology·Ivar RønnestadM Leonor Cancela
Dec 7, 2007·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Elena ValassiFrancesco Cavagnini
Jan 29, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hiroyuki KaiyaSuraj Unniappan
Aug 30, 2008·Physiological Genomics·Robert E DrewBarrie D Robison
Nov 26, 2008·General and Comparative Endocrinology·Navpreet Amole, Suraj Unniappan

❮ Previous
Next ❯

Citations

May 21, 2015·Fish Physiology and Biochemistry·Camila DalmolinLuis Fernando Marins
Apr 12, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A M BlancoM J Delgado
Oct 16, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yuko KamisakaIvar Rønnestad
Aug 21, 2013·Behavioural Brain Research·Michael NguyenAllan V Kalueff
Oct 30, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zenith Gaye A OrozcoSoichi Watanabe
Nov 3, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Tiziano VerriAlessandro Romano
Dec 7, 2017·Frontiers in Endocrinology·Vanessa H Quinlivan, Steven A Farber
Mar 16, 2019·Frontiers in Endocrinology·Juan Ignacio BertucciSuraj Unniappan
May 2, 2017·Frontiers in Endocrinology·Ivar RønnestadHélène Volkoff
Mar 17, 2020·General and Comparative Endocrinology·Cody Petro-SakumaAndre P Seale

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.