The effect of feeding Bt MON810 maize to pigs for 110 days on intestinal microbiota.

PloS One
S G BuzoianuPeadar G Lawlor

Abstract

To assess the effects of feeding Bt MON810 maize to pigs for 110 days on the intestinal microbiota. Forty male pigs (∼40 days old) were blocked by weight and litter ancestry and assigned to one of four treatments; 1) Isogenic maize-based diet for 110 days (Isogenic); 2) Bt maize-based diet (MON810) for 110 days (Bt); 3) Isogenic maize-based diet for 30 days followed by a Bt maize-based diet for 80 days (Isogenic/Bt); 4) Bt maize-based diet for 30 days followed by an isogenic maize-based diet for 80 days (Bt/Isogenic). Enterobacteriaceae, Lactobacillus and total anaerobes were enumerated in the feces using culture-based methods on days 0, 30, 60 and 100 of the study and in ileal and cecal digesta on day 110. No differences were found between treatments for any of these counts at any time point. The relative abundance of cecal bacteria was also determined using high-throughput 16 S rRNA gene sequencing. No differences were observed in any bacterial taxa between treatments, with the exception of the genus Holdemania which was more abundant in the cecum of pigs fed the isogenic/Bt treatment compared to pigs fed the Bt treatment (0.012 vs 0.003%; P≤0.05). Feeding pigs a Bt maize-based diet for 110 days did not affect counts of any o...Continue Reading

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Citations

Sep 12, 2013·Nature Biotechnology·Laura DeFrancesco
Feb 24, 2016·Computational and Structural Biotechnology Journal·Simon DeuschJana Seifert
Sep 4, 2014·Journal of Animal Science·A L Van Eenennaam, A E Young
Jul 21, 2017·Journal of Animal Science·A L Van Eenennaam, A E Young
May 21, 2017·Applied and Environmental Microbiology·Ursula M McCormackPeadar G Lawlor
Jan 11, 2020·Frontiers in Plant Science·Paula A GiraldoKevin F Smith
Jun 21, 2020·Journal of the Science of Food and Agriculture·Ruqing ZhongHongfu Zhang
May 24, 2020·Animals : an Open Access Journal From MDPI·Stefan G BuzoianuFabio Vannucci
Dec 22, 2017·Journal of Agricultural and Food Chemistry·Penggao LiRong Xiao

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Datasets Mentioned

BETA
ERP001333

Methods Mentioned

BETA
PCR
electrophoresis
454 Genome

Software Mentioned

MOTHUR
SAS
QIIME

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