The Effect of Feeding Cocoa Powder and Lactobacillus rhamnosus on the Composition and Function of Pig Intestinal Microbiome

Current Developments in Nutrition
Gloria I Solano-AguilarCindy D Davis

Abstract

Dietary habits have been linked with variability of gut microbiota composition and disease risk. The aim of this study was to evaluate the effect of feeding a cocoa powder with or without a probiotic on the composition and function of the fecal microbiome of pigs. Four groups of 8 pigs each were fed a standard growth diet supplemented with cocoa powder, Lactobacillus rhamnosus (LGG), cocoa powder + LGG, or an equal amount of fiber similar to that found in cocoa powder (control group). Fecal samples were collected prior to and 4 wk after initiation of the dietary intervention. Microbiota composition was determined after amplification of the first 2 variable regions of the 16S ribosomal DNA (rDNA). Predictions of metagenomic function were calculated using 16S rDNA sequence data through Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). After 4 wk of treatment, bacterial abundance analysis demonstrated a prebiotic effect of cocoa powder on endogenous Bifidobacteriaceae and Lactobacillaceae and increased abundance of saccharolytic butyrate-producing bacteria like Roseburia. An increased bacterial evenness, Shannon diversity index, and diverse metabolic profile were detected in microbiomes of...Continue Reading

References

Oct 3, 2006·International Journal of Systematic and Evolutionary Microbiology·Sylvia H DuncanHarry J Flint
Dec 22, 2006·Nature·Ruth E LeyJeffrey I Gordon
Nov 2, 2007·Alimentary Pharmacology & Therapeutics·H M HamerR-J Brummer
May 24, 2008·Science·Ruth E LeyJeffrey I Gordon
May 22, 2010·Chemistry & Biodiversity·Patrick GillevetEce A Mutlu
Jun 16, 2010·Journal of the Science of Food and Agriculture·Ronald L PriorJess Reed
May 18, 2011·BMC Microbiology·Regina LamendellaDaniel B Oerther
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Dec 24, 2011·Nucleic Acids Research·Victor M MarkowitzNikos C Kyrpides
Jun 6, 2012·Archives of Biochemistry and Biophysics·Malen Massot-CladeraFrancisco J Pérez-Cano
Jul 4, 2012·Critical Reviews in Food Science and Nutrition·J M Landete
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·A Bähr, E Wolf
Oct 13, 2012·Gut Microbes·Gunnar Loh, Michael Blaut
Dec 12, 2012·BioTechniques·Masoumeh Sikaroodi, Patrick M Gillevet
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Nov 28, 2013·GigaScience·Yoshiki Vázquez-BaezaRob Knight
Jul 23, 2014·The Journal of Microbiology·Edward Alain B PajarilloDae-Kyung Kang
Dec 3, 2014·Applied and Environmental Microbiology·Szymon P SzafranskiIrene Wagner-Döbler
Feb 20, 2015·Gut Microbes·Bonnie P YoumansSarah K Highlander
Mar 27, 2015·The British Journal of Nutrition·Charlotte E MillsJeremy P E Spencer
Sep 24, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juana I MoseleMaria-José Motilva
Feb 16, 2016·Journal of Agricultural and Food Chemistry·Damiano MagistrelliFabia Rosi
Apr 29, 2016·Genome Medicine·Jason Lloyd-PriceCurtis Huttenhower
Jun 14, 2016·The Journal of Allergy and Clinical Immunology·Supinda BunyavanichJose C Clemente
Aug 6, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Luisa Z MorenoAndrea M Moreno
Aug 26, 2016·The Journal of Nutritional Biochemistry·Karen M StratAndrew P Neilson
Aug 26, 2016·Clinical and Translational Gastroenterology·Dae J KangJasmohan S Bajaj
Sep 13, 2016·Molecular Metabolism·Torsten P M ScheithauerDaniël H van Raalte
Dec 21, 2016·Frontiers in Microbiology·Swadha AnandSharmila S Mande

❮ Previous
Next ❯

Citations

Jan 31, 2020·Journal of Animal Science·Andrew R WilliamsAlexandra Blanchard
Mar 16, 2021·Frontiers in Immunology·Audrey I S Andersen-CivilAndrew R Williams

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.