The effect of FFAR1 on pioglitazone-mediated attenuation of palmitic acid-induced oxidative stress and apoptosis in βTC6 cells

Metabolism: Clinical and Experimental
Ximei ShenXiuhui Cai

Abstract

We sought to determine whether free fatty acid receptor 1 (FFAR1), a receptor for free fatty acids on the β-cell membrane, can mediate the pioglitazone (PIO)-attenuating effect on lipoapoptosis in β cells and its relationship to oxidative stress. The glucose-sensitive mouse beta pancreatic cell line βTC6 was used to investigate the effect of FFAR1 on PIO-attenuating palmitic acid (PA)-induced oxidative stress and apoptosis. (1) PIO reduced PA-induced lipoapoptosis in β cells and upregulated the expression of FFAR1 at the mRNA and protein levels in a dose- and time-dependent manner. Silencing of FFAR1 expression was shown to weaken the protective effect of PIO on PA-induced lipoapoptosis in βTC6 cells; while lentiviral-mediated overexpression of FFAR1 was shown to enhance the protective effect of PIO against lipoapoptosis in β cells. (2) Downregulation of FFAR1 expression reduced the attenuating effect of PIO on the expression of NAPDH oxidase subunit p47(phox), Bax, cleaved caspase 3, and the production of reactive oxygen specific (ROS) induced by lipotoxicity, thereby preventing the upregulation of the expression of bcl-2. Inducing the overexpression of FFAR1 enhanced the anti-oxidative stress effect of PIO. Similarly, these e...Continue Reading

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Citations

Jul 14, 2014·Metabolism: Clinical and Experimental·Morgan L MoonGregory G Freund
Jun 15, 2016·Chemical Reviews·Graeme MilliganBrian D Hudson
Feb 19, 2017·Journal of Cellular Physiology·Eugene LeeHyun Soon Lee
Nov 10, 2017·Antioxidants & Redox Signaling·Carlota RecioDavid R Greaves
Jul 4, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng LiJianbin He
Apr 11, 2020·Pharmacological Reports : PR·Eloisa Aparecida Vilas-BoasLeticia Prates Roma
Apr 10, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Baicheng MaLei Yang
May 1, 2021·International Journal of Molecular Sciences·Jan ŠrámekJan Kovář

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