PMID: 7170703Jan 1, 1982

The effect of fibrin on endothelial cell migration in vitro

Tissue & Cell
R R Schleef, C Birdwell

Abstract

Endothelial cells are known to migrate and come into contact with fibrin during numerous physiological processes, such as in wound healing and in tumor growth. The present study was initiated to investigate the effect of fibrin on endothelial cell migration in vitro. Endothelial cell migration was assayed by wounding confluent monolayers of bovine aortic endothelial cells with a razor blade and counting the number of cells crossing the wound per unit time. Wound-induced proliferation of endothelial cells was inhibited by mitomycin C-treatment without affecting endothelial cell migration, indicating that in this assay migration could be measured independent of proliferation. Migration of endothelial cells in vitro was inhibited by fibrin in a concentration dependent manner. Endothelial cell migration under fibrin was further reduced by plasminogen depletion of the serum, and fibrin still inhibited the migration of mitomycin C-treated endothelial cells. Kadish et al. (Tissue and Cell, 11, 99, 1979) previously reported that fibrin did not affect EC migration in vitro. The inability to inhibit EC migration with fibrin appears to be due to their assay system which employed agarose, since pre-treating the wounded monolayer with agaro...Continue Reading

References

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Citations

Nov 1, 1992·Journal of Cellular Biochemistry·B L Coomber
Jul 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A M Schor, S L Schor
May 25, 1984·Journal of Immunological Methods·J L Obeso, R Auerbach
Jan 1, 1991·Pharmacology & Therapeutics·R Eisenstein
Sep 11, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Thao-Nguyen NguyenJ Thomas Pento
Dec 10, 2002·Journal of Vascular and Interventional Radiology : JVIR·Masao HamuroNorihumi Nishida
Jan 1, 1997·Journal of Vascular and Interventional Radiology : JVIR·E A SpragueJ C Palmaz
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·A Sahni, C W Francis
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·A SahniC W Francis
Oct 1, 1990·Analytical Biochemistry·K JozakiD L Kreutzer
Mar 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Koichi UnoTarl W Prow
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·K L KaplanS Solomon
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R I LevinE A Jaffe
May 18, 1999·The Journal of Biological Chemistry·A SahniC W Francis

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