The Effect of Fixed Orthodontic Appliances and Fluoride Mouthwash on the Oral Microbiome of Adolescents - A Randomized Controlled Clinical Trial

PloS One
Jessica E KoopmanEgija Zaura

Abstract

While the aesthetic effect of orthodontic treatment is clear, the knowledge on how it influences the oral microbiota and the consequential effects on oral health are limited. In this randomized controlled clinical trial we investigated the changes introduced in the oral ecosystem, during and after orthodontic treatment with fixed appliances in combination with or without a fluoride mouthwash, of 10-16.8 year old individuals (N = 91). We followed several clinical parameters in time, in combination with microbiome changes using next-generation sequencing of the bacterial 16S rRNA gene. During the course of our study, the oral microbial community displayed remarkable resilience towards the disturbances it was presented with. The effects of the fluoride mouthwash on the microbial composition were trivial. More pronounced microbial changes were related to gingival health status, orthodontic treatment and time. Periodontal pathogens (e.g. Selenomonas and Porphyromonas) were highest in abundance during the orthodontic treatment, while the health associated Streptococcus, Rothia and Haemophilus gained abundance towards the end and after the orthodontic treatment. Only minor compositional changes remained in the oral microbiome after th...Continue Reading

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Citations

Mar 8, 2017·Journal of Clinical Periodontology·Alex MiraM A Curtis
May 16, 2018·International Journal of Oral Science·Peng XianLiao Ga
Sep 29, 2019·Journal of Dental Research·M A PayneM A Curtis
Oct 5, 2019·Dentistry Journal·Sandra PetrauskieneGintare Zemgulyte
Mar 11, 2021·Periodontology 2000·A M Marije KaanEgija Zaura
Aug 28, 2021·Microorganisms·Yukiko YanoChristian C Abnet
Sep 20, 2021·Orthodontics & Craniofacial Research·Isaac ChenAndrew H Jheon

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Datasets Mentioned

BETA
SRP055565

Software Mentioned

PAST
Quantitative Insights Into Microbial Ecology ( QIIME )
QIIME
Megablast
SPSS Statistics

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