The effect of fructan on membrane lipid organization and dynamics in the dry state

Biophysical Journal
Ingrid J VereykenB de Kruijff

Abstract

Fructans are a group of fructose-based oligo- and polysaccharides, which appear to be involved in membrane preservation during dehydration by interacting with the membrane lipids. To get further understanding of the protective mechanism, the consequences of the fructan-membrane lipid interaction for the molecular organization and dynamics in the dry state were studied. POPC and DMPC were investigated in the dry state by (2)H, (31)P NMR, and Fourier transform infrared spectroscopy using two types of fructan and dextran. The order-disorder transition temperature of dry POPC was reduced by 70 degrees C in the presence of fructan. Fructan increased the mobility of the acyl chains, but immobilized the lipid headgroup region. Most likely, fructans insert between the headgroups of lipids, thereby spacing the acyl chains. This results in a much lower phase transition temperature. The headgroup is immobilized by the interaction with fructan. The location of the interaction with the lipid headgroup is different for the inulin-type fructan compared to the levan-type fructan, since inulin shows interaction with the lipid phosphate group, whereas levan does not. Dextran did not influence the phase transition temperature of dry POPC showing ...Continue Reading

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Apr 30, 2003·Biophysical Journal·Ingrid J VereykenBen de Kruijff

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Citations

Mar 18, 2009·Cellular and Molecular Life Sciences : CMLS·David P LivingstonArnd G Heyer
Nov 26, 2010·Archives of Microbiology·M Lourdes Velázquez-HernándezJuan J Valdez-Alarcón
Mar 14, 2008·Pharmaceutical Research·J-P AmorijW L J Hinrichs
Nov 26, 2010·Plant & Cell Physiology·Ming-Der ShihYue-Ie C Hsing
Jul 14, 2007·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jørgen de JongeHenderik W Frijlink
Feb 9, 2006·Systematic and Applied Microbiology·Clarissa Schwab, Michael G Gänzle
Oct 3, 2015·Carbohydrate Polymers·Maarten A MensinkWouter L J Hinrichs
Oct 29, 2003·Biophysical Journal·Ingrid J VereykenBen de Kruijff
Sep 1, 2015·Frontiers in Plant Science·Emerik De SadeleerWim Van den Ende
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Leonie VogtPaul De Vos
Nov 21, 2007·Journal of Liposome Research·Jan WilschutHenderik W Frijlink
Apr 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·De-Jiang PangYuan-Feng Zou
Apr 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Jian JinIzumi Ichinose

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