The effect of gallium nitride on long-term culture induced aging of neuritic function in cerebellar granule cells

Biomaterials
Chi-Ruei Chen, Tai-Horng Young

Abstract

Gallium nitride (GaN) has been developed for a variety of microelectronic and optical applications due to its unique electric property and chemical stability. In the present study, n-type and p-type GaN were used as substrates to culture cerebellar granule neurons to examine the effect of GaN on cell response for a long-term culture period. It was found that GaN could rapidly induce cultured neurons to exhibit a high phosphorylated Akt level after 20h of incubation. It was assumed that the anti-apoptotic effect of Akt phosphorylation could be correlated with cell survival, neurite growth and neuronal function for up to 35 days of incubation. Morphological studies showed GaN induced larger neuronal aggregates and neurite fasciculation to exhibit a dense fiber network after 8 days of incubation. Western blot analysis and immunocytochemical characterization showed that GaN still exhibited the expression of neurite growth and function, such as high levels of GAP-43, synapsin I and synaptophysin even after 35 days of incubation. In addition, survival of cerebellar granule neurons on GaN was improved by the analysis of lactate dehydrogenase (LDH) release from damaged cells. These results indicated that neuronal connections were forme...Continue Reading

References

Jan 1, 1986·Annals of the New York Academy of Sciences·L I BinderL I Rebhun
Apr 16, 1987·Nature·M Bähler, P Greengard
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Jan 1, 1984·Cell Motility·M J KatzL J Gilbert
May 1, 1995·Neurobiology of Aging·J W Mandell, G A Banker
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·L S ChinP Greengard
Feb 1, 1997·Trends in Neurosciences·L I Benowitz, A Routtenberg
Mar 5, 1999·Journal of Neuroscience Methods·S MartinoiaM Grattarola
Dec 2, 1999·Experimental Cell Research·E S Kandel, N Hay
Mar 21, 2001·FEBS Letters·E HajduchH S Hundal
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Zeck, P Fromherz
Mar 7, 2006·Biomaterials·Tai-Horng Young, Chi-Ruei Chen
Mar 17, 2012·Seminars in Immunology·Steven A Corfe, Christopher J Paige

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Citations

Jul 12, 2013·ACS Applied Materials & Interfaces·Lauren E BainAlbena Ivanisevic
Oct 1, 2013·ACS Applied Materials & Interfaces·Jingying LiChen Wang
Aug 19, 2009·Tissue Engineering. Part C, Methods·Evie L PapadopoulouEmmanuel Stratakis
Oct 25, 2011·Acta Biomaterialia·Scott A JewettAlbena Ivanisevic
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·L E BainA Ivanisevic
Mar 5, 2014·Acta Biomaterialia·Lauren E BainAlbena Ivanisevic
Jan 8, 2015·Nanoscale·Lauren E BainAlbena Ivanisevic
Sep 30, 2014·Colloids and Surfaces. B, Biointerfaces·Jingying LiChen Wang

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