The effect of Ganoderma lucidum extract on immunological function and identify its anti-tumor immunostimulatory activity based on the biological network

Scientific Reports
Ruo-Lin ZhaoYu-Min He

Abstract

Ganoderma lucidum extract (GLE) has shown positive effects for tumor treatment. However, the molecular mechanism of GLE treatment is unknown. In this study, a Hepa1-6-bearing C57 BL/6 mouse model was established to explore the anti-tumor and immunostimulatory activity of GLE treatment. The results showed that GLE effectively inhibited tumor growth without hepatic/renal toxicity and bone marrow suppression, and might enhancing immunological function. Based on the mRNA profiles of GLE treated and untreated mice, 302 differentially expressed (DE) mRNAs were identified and 6 kernel mRNAs were identified from the established protein-protein interaction (PPI) network. Quantitative RT-PCR and western-blot analysis indicated that 6 mRNAs have had statistically significant differences between the GLE treated and untreated mice. Furthermore, four kernel pathways were isolated from the KEGG-Target network, including the Jak-STAT signaling pathway, T cell receptor signaling pathway, PI3K-Akt signaling pathway, and cytokine-cytokine receptor interaction. Western-blot and cytokine detection results demonstrated that GLE suppressed growth and proliferation of tumors by the Jak-STAT signaling pathway, T cell receptor signaling pathway and PI3K...Continue Reading

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Citations

Jan 27, 2021·Journal of Integrative Medicine·Yue-Jiao ShiZhen-Hao Li
May 6, 2021·Journal of Fungi·Jelena ŽivkovićJasmina Glamočlija
Sep 30, 2021·Natural Product Research·Mario ForzanMaurizio Mazzei
Feb 4, 2022·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Beibei LuJiaheng Zhang

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Datasets Mentioned

BETA
GSE117503
PMDA179

Methods Mentioned

BETA
flow cytometry
FACS
chip
biopsy
fluorescence activated cell sorting
PCR
protein assay

Software Mentioned

R package
SPSS
R
GenePix Pro
ClusterONE
DAVID
Image [UNK]

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