PMID: 4898022Aug 1, 1969Paper

The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization

The Biochemical Journal
J O Bishop

Abstract

1. The rate of RNA-DNA hybridization was studied under conditions of RNA excess, with RNA synthesized in vitro. The initial rate of the reaction was proportional to the initial RNA concentration. Throughout the observed course of the reaction there was a linear relationship between the reciprocal of the amount of RNA hybridized/mug. of DNA and the reciprocal of time. The slope of the reciprocal plot was inversely proportional to the initial RNA concentration. 2. A comparison was made of the hybridization of DNA from Escherichia coli and from bacteriophages T4 and lambda with homologous RNA. The initial rate of hybridization was inversely proportional to the genetic complexity of the hybridizing system. The slope of the reciprocal-time plot was directly proportional to genetic complexity. These results are interpreted to indicate that the rate of hybridization reflects the mean concentration of the various unique RNA species in a preparation.

Citations

Sep 16, 1974·European Journal of Biochemistry·R A FlavellE J Birfelder
Feb 23, 1972·Nature: New Biology·J O BishopC Baglioni
Oct 8, 2005·Nature Medicine·Edwin Southern
Aug 1, 1975·Journal of Neurochemistry·M WintzerithP Mandel
Nov 8, 1969·Nature·J O Bishop
Jun 1, 1975·Bulletin of Mathematical Biology·J M Reiner
Sep 16, 1974·European Journal of Biochemistry·R A FlavellP Borst
May 15, 1974·European Journal of Biochemistry·H FouquetH W Sauer
Jul 19, 1974·Nature·J O BishopM Richardson
Jan 1, 1974·Chromosoma·K W JonesG Corneo
Jul 1, 1977·European Journal of Biochemistry·A E SippelG Schütz
Jun 13, 1973·Nature: New Biology·L J Grady, W P Campbell
Dec 6, 1976·Chromosoma·L SinghK W Jones
Sep 6, 1976·Biochimica Et Biophysica Acta·J M TaylorJ Summers
Dec 19, 1975·Biochimica Et Biophysica Acta·C Shalitin, I Fischer
Feb 1, 1973·Journal of Virology·W S Hayward, H Hanafusa
Dec 1, 1974·Developmental Biology·H ToblerO Kuhn
Sep 15, 1973·Journal of Molecular Biology·S G ClarksonV Serra
Mar 13, 1973·Biochemistry·G B SpiegelmanH O Halvorson

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