PMID: 7409271Jun 20, 1980Paper

The effect of glucose and 2-deoxy-D-glucose on the monodeiodination of thyroxine by human skin fibroblasts in culture (author's transl)

Nihon Naibunpi Gakkai zasshi
S Ishikawa, T Saito


The effect of glucose and 2-deoxy-D-glucose on the extrathyroidal monodeiodination of thyroxine (T4) to 3, 5, 3'-triiodothyronine (T3) and 3, 3', 5'-triiodohyronine (reverse T3;rT3) was studied in human skin fibroblasts in culture. When the fibroblasts were maintained in glucose-free Eagle's MEM fortified with 3% fetal calf serum, intracellular T4 was unchanged, intracellular T3 decreased, and intracellular rT3 markedly increased as compared with those in Eagle's MEM containing 3% fetal calf serum and 100 mg/dl glucose. In this study, the ratio of T3 to T4 fell, and the ratio of rT3 to T4 and rT3 to T3 rose remarkably in fibroblasts in glucose-deprived media. When the fibroblasts were maintained in Eagle's MEM containing 3% fetal calf serum and various concentration of 2-deoxy-D-glucose, intracellular concentrations of thyroid hormone were decreased as compared with those in 2-deoxy-D-glucose-free control medium. The ratio of rT3 to T4 and rT3 to T3, however, augmented although the ratio of T3 to T4 did not change in fibroblasts in the media with 2-deoxy-D-glucose. Cellular energetic deprivation derived from the glucose-free medium and the medium containing 2-deoxy-D-glucose influenced the extrathyroidal monodeiodination of T4 ...Continue Reading

Related Concepts

Deoxy Sugars
Glucose, (beta-D)-Isomer
Reverse Triiodothyronine
Culture Techniques

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Laryngeal Neoplasms

Laryngeal Neoplasms occur in the Larynx and are typically associated with smoking and alcohol consumption. Discover the latest research on Laryngeal Neoplasms here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.