PMID: 9545023Apr 17, 1998Paper

The effect of glucose concentration on insulin-induced 3T3-L1 adipose cell differentiation

Obesity Research
A M Gagnon, A Sorisky

Abstract

We examined the effect of glucose concentration on insulin-induced 3T3-L1 adipose cell differentiation. Oil Red O staining of neutral lipid, cellular triglyceride mass, and glycerol phosphate dehydrogenase (GPDH) activity, were greater in 3T3-L1 cells cultured at 5 mM vs. 25 mM glucose. GPDH activity was 2- to 4-fold higher at 5 mM vs. 25 mM glucose over a range of insulin concentrations (0.1 to 100 nM). Insulin-stimulated tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) was 1.7-fold greater, and insulin-stimulated phosphoinositide 3-kinase association with IRS-1 was 2.3-fold higher, at 5 mM vs. 25 mM glucose. These effects of glucose were not caused by alterations in IRS-1 mass or cell-surface insulin binding. In preadipose cells at 5 mM glucose, expression of the leukocyte antigen-related (LAR) protein tyrosine phosphatase (negative regulator of insulin signaling) was 63% of the level at 25 mM glucose. Our data demonstrate that glucose concentration affects insulin-induced 3T3-L1 adipose cell differentiation as well as differentiation-directed insulin signaling pathways. Alterations in LAR expression potentially may be involved in modulating these responses.

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Accili, S I Taylor
Apr 19, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·D Mikac-DevićK Bosković
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·W C YehS L McKnight
Jul 6, 1995·Biochemical and Biophysical Research Communications·K TomiyamaY Watanabe
Jan 1, 1995·Annual Review of Biochemistry·O A MacDougald, M D Lane
Jul 1, 1995·The National Medical Journal of India·A Misra
Feb 10, 1995·The Journal of Biological Chemistry·D T KulasR A Mooney
Feb 1, 1994·Current Opinion in Genetics & Development·M F White
Jun 28, 1996·The Journal of Biological Chemistry·C SchubertB Draznin
Apr 1, 1996·Current Opinion in Cell Biology·C L Carpenter, L C Cantley

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Citations

Sep 27, 2001·Journal of Cellular Physiology·A GagnonA Sorisky
May 5, 2016·Adipocyte·Sara Palacios-OrtegaFermín I Milagro
Mar 31, 2015·The International Journal of Biochemistry & Cell Biology·Aleix Gavaldà-NavarroTeresa Mampel
Jun 1, 2000·Obesity Research·A BellA Sorisky
Oct 17, 2013·Diabetes·Guenther BodenSalim Merali
Jan 28, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Torri E RinkerJohnna S Temenoff
Feb 8, 2018·Scientific Reports·Duy Trong Vien DiepKwang-Hoon Chun
Jan 18, 2003·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·C S SolomonM L Goalstone
Jul 4, 2008·American Journal of Physiology. Cell Physiology·Joanne T M TanStephen M Twigg
Sep 21, 2000·The Proceedings of the Nutrition Society·J A Martinez
Sep 5, 2001·American Journal of Human Biology : the Official Journal of the Human Biology Council·Ping AnD.C. Rao
Sep 5, 2001·American Journal of Human Biology : the Official Journal of the Human Biology Council·Peter T. KatzmarzykClaude Bouchard
Mar 19, 2020·Cells·Justin Hou Ming Yung, Adria Giacca
Mar 22, 2013·The Journal of Biological Chemistry·Soroush TahmasebiXiang-Jiao Yang
Nov 13, 2004·The Journal of Biological Chemistry·Ying LinPhilipp E Scherer
Apr 15, 2011·The Journal of Experimental Biology·Vikas Kumar Roy, Amitabh Krishna

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