PMID: 3140799Aug 15, 1988

The effect of glycerol on the activity of beta-glucosidase from Botryodiplodia theobromae Pat

The Biochemical Journal
G M Umezurike


1. In the activity of the high-Mr beta-glucosidase A (beta-D-glucoside glucohydrolase, EC obtained from culture filtrates of Botryodiplodia theobromae Pat. on o-nitrophenyl beta-D-glucopyranoside as substrate, both Vmax. and Km increased non-linearly with increasing concentration of glycerol, and the Vmax./Km(app.) ratio decreased non-linearly with increasing concentration of glycerol. 2. No increase in rate was observed with phenyl beta-D-glucopyranoside as substrate in the presence of up to 250 mM-glycerol, indicating that glucosylation is rate-limiting with this substrate. 3. With o-nitrophenyl beta-D-glucopyranoside, p-nitrophenyl beta-D-glucopyranoside and phenyl beta-D-glucopyranoside as substrates, kappa cat. values of 793.7 s-1, 62.8 s-1 and 5.4 s-1 respectively were calculated. 4. With o-nitrophenyl beta-D-glucopyranoside and phenyl beta-D-glucopyranoside as substrate, alpha-deuterium kinetic isotope effects of 1.9 +/- 0.03 and 1.01 +/- 0.01 respectively were found; in the presence of 200 mM-glycerol the values were 1.21 +/- 0.03 and 1.02 +/- 0.01 respectively. 5. In the presence of a large excess of o-nitrophenyl beta-D-glucopyranoside [( S] = 35.7 Km), the amount of o-nitrophenol and also of the transglucos...Continue Reading


Oct 20, 1992·Biochemistry·J B Kempton, Stephen G Withers
Feb 1, 2005·Carbohydrate Research·David J Vocadlo, Stephen G Withers

Related Concepts

2-nitrophenol, sodium salt
Emulsion beta-D-Glucosidase
Glucose, (beta-D)-Isomer

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