The effect of growth at low temperature on the activity and expression of the uncoupling protein in Acanthamoeba castellanii mitochondria

FEBS Letters
W JarmuszkiewiczF E Sluse

Abstract

Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP could be a cold response protein in unicellulars. In mitochondria isolated from an amoeba batch culture grown temporarily at low temperature (6 degrees C), the content of AcUCP was increased and correlated with an increase in the linoleic acid (LA)-stimulated UCP-mediated carboxyatractyloside-resistant state 4 respiration, as compared to a control culture (routinely grown at 28 degrees C). Moreover, the cytochrome pathway activity was found to be insensitive to the cold exposure of amoeba cells, as indicated by respiration and membrane potential measurements as well as by an absence of change in the adenine nucleotide translocator and cytochrome oxidase expression levels. Furthermore, in mitochondria from the low-temperature-grown cells, at fixed LA concentration, the increased contribution of AcUCP activity to total mitochondrial phosphorylating respiration accompanied by lower coupling parameters was found, as was...Continue Reading

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Citations

Apr 28, 2011·Journal of Bioenergetics and Biomembranes·Andrzej Woyda-PloszczycaWieslawa Jarmuszkiewicz
May 11, 2011·Journal of Bioenergetics and Biomembranes·Sergio Guerrero-CastilloSalvador Uribe-Carvajal
Jan 20, 2010·FEBS Letters·Wieslawa JarmuszkiewiczIzabela Koszela-Piotrowska
Sep 1, 2006·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Felix C MarkHans O Pörtner
Dec 23, 2009·Biochimica Et Biophysica Acta·Wieslawa JarmuszkiewiczFrancis E Sluse
Nov 12, 2005·Bioscience Reports·Denise Loli, José Eduardo P W Bicudo
Apr 17, 2007·Journal of Bioenergetics and Biomembranes·Malgorzata CzarnaWieslawa Jarmuszkiewicz
Mar 6, 2007·Journal of Bioenergetics and Biomembranes·Aleksandra SwidaWieslawa Jarmuszkiewicz

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