PMID: 6160129Nov 1, 1980Paper

The effect of histochemical methylation on the phosphate groups of nucleic acids: interpretation of the absence of nuclear basophilia

The Histochemical Journal
P Prentø

Abstract

The effect of hot methylation (hydrochloric acid-methanol)) on nuclear stainability was investigated in order to determine whether the progressive loss of basophilia is due to methylation of the diester phosphate groups of nucleic acid. DNA spots on filter paper were unchanged in their stainability towards Toluidine Blue even after methylation for 4 days, while RNA, chondroitin sulphate and hyaluronic acid lost their affinity for this dye after 4 h methylation. In formalin-fixed sections, methylation for 4 h led to a loss of nuclear basophilia. There was no concomitant increase in nuclear relative to cytoplasmic stainability with Fast Green FCF at pH 9, as judged from the use of a comparison eyepiece, evaluation of colour transparencies or by microspectrophotometry. In contrast, extraction with trichloroacetic acid prior to or after methylation led to a much improved Fast Green staining of nuclei, comparable to the staining obtainable after treatment with trichloroacetic acid alone. In conclusion, there is no evidence that hot hydrochloric acid-methanol, as used in histochemistry, methylates the diester phosphate groups of nucleic acids. The loss of nuclear basophilia can be explained as a result of the excess positive charge o...Continue Reading

References

Jun 29, 1973·Histochemie. Histochemistry. Histochimie·P N Marshall, R W Horobin
May 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A D DeitchR M Richart
Feb 1, 1969·The Histochemical Journal·R Kodousik
Mar 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E R FISHER, R D LILLIE
Mar 1, 1958·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R D LILLIE
Jul 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Y TERNER
Oct 1, 1953·Proceedings of the National Academy of Sciences of the United States of America·M Alfert, I I Geschwind

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Citations

Jan 13, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·J A Kiernan
Apr 6, 1999·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K Conway, J A Kiernan

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