The Effect of Human Mesenchymal Stem Cells Derived from Wharton's Jelly in Spinal Cord Injury Treatment Is Dose-Dependent and Can Be Facilitated by Repeated Application

International Journal of Molecular Sciences
Petr KrupaPavla Jendelová

Abstract

Human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) were used for the treatment of the ischemic-compression model of spinal cord injury in rats. To assess the effectivity of the treatment, different dosages (0.5 or 1.5 million cells) and repeated applications were compared. Cells or saline were applied intrathecally by lumbar puncture for one week only, or in three consecutive weeks after injury. Rats were assessed for locomotor skills (BBB, rotarod, flat beam) for 9 weeks. Spinal cord tissue was morphometrically analyzed for axonal sprouting, sparing of gray and white matter and astrogliosis. Endogenous gene expression (Gfap, Casp3, Irf5, Cd86, Mrc1, Cd163) was studied with quantitative Real-time polymerase chain reaction (qRT PCR). Significant recovery of functional outcome was observed in all of the treated groups except for the single application of the lowest number of cells. Histochemical analyses revealed a gradually increasing effect of grafted cells, resulting in a significant increase in the number of GAP43+ fibers, a higher amount of spared gray matter and reduced astrogliosis. mRNA expression of macrophage markers and apoptosis was downregulated after the repeated application of 1.5 million cells. We...Continue Reading

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Citations

May 28, 2019·International Journal of Molecular Sciences·Loubna MaziniGabriel Malka
Jun 20, 2020·Journal of Cellular Physiology·Hossein AbbaszadehKarim Shamsasenjan
Oct 20, 2018·International Journal of Molecular Sciences·Pavla Jendelova
Dec 12, 2018·Neural Regeneration Research·Yana O MukhamedshinaAlbert A Rizvanov
Sep 25, 2019·International Journal of Molecular Sciences·Milada ChudickovaSarka Kubinova
Apr 22, 2019·Neurochemical Research·Dasa CizkovaMichel Salzet
Oct 23, 2020·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Petr KrupaIgor A Lavrov
Jan 5, 2021·The Journal of Obstetrics and Gynaecology Research·Hezhu WangYuquan Zhang
Nov 11, 2020·Biomedicines·Petr KrupaLucia Machova Urdzikova
Apr 16, 2021·Advanced Science·Anna AndrzejewskaMiroslaw Janowski
Jul 24, 2021·Frontiers in Cell and Developmental Biology·Eva SykovaSarka Kubinova

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

StepOnePlus
Sistat
ImageJ
Sigmastat

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