PMID: 15374472Nov 1, 1990Paper

The effect of idebenone on the lateral mobility of proteins in hepatocyte plasma membrane of old rats as revealed by fluorescence recovery after photobleaching (FRAP) technique using an endogenous label

Archives of Gerontology and Geriatrics
I Zs -NagyK Kitani

Abstract

The average lateral diffusion constant of proteins (D) in the cell membrane of hepatocytes has been measured in liver smears by fluorescence recovery after photobleaching (FRAP) based on the so-called peroxide-induced autofluorescence (PIAF) deriving from the oxidation of riboflavin bound to membrane proteins. It has been shown before that D displays a significant negative linear age-correlation. The in vivo effect of idebenone was tested on this parameter. Old (23.7 months at killing) male Fischer 344 rats received 50 mg of the drug per kg body weight per os through gastric tube, suspended in 5% gum arabic solution (Verum I group) or the same dose and form of idebenone completed by 80 mg centrophenoxine per kg body weight (Verum II group) for 35 days. D was measured on a double blind basis in hepatocyte plasma membrane of the Verum or Placebo (5% gum arabic, only) groups. A significant increase of D (29.6%) was induced by idebenone in the Verum I group; nevertheless, even the Placebo group displayed a moderate, but statistically significant increase of D (10.7%). The Verum II group showed a total of 39.6% increase in the value of D. The loss of body weight being characteristic for these animals at the age of around 2 years was...Continue Reading

References

Dec 20, 1979·Biochimica Et Biophysica Acta·R J Cherry
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerE L Elson
Apr 22, 1988·Biochimica Et Biophysica Acta·M NokuboM Ohta
Jan 1, 1974·Annual Review of Biophysics and Bioengineering·M Edidin
Oct 1, 1982·Biophysical Journal·J YguerabideE E Yguerabide
Nov 1, 1990·Archives of Gerontology and Geriatrics·I Zs -Nagy

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Citations

Sep 1, 1994·Archives of Gerontology and Geriatrics·K Kitani
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·M J Saxton, K Jacobson
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J Lippincott-SchwartzK Hirschberg

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