PMID: 15374473Nov 1, 1990Paper

The effect of idebenone on the passive K+ and Rb+ permeability of the brain cell membranes of CFY rats as revealed by the Rb+-discrimination ratio

Archives of Gerontology and Geriatrics
G LustyikI Zs -Nagy

Abstract

Rubidium (Rb(+)) uptake and release of brain cortical neurons of adult (12-15 month old), normal, female CFY rats were studied in placebo- and idebenone-treated animals. Treatment period was up to 5 weeks, with 50 mg/kg bw/day idebenone (oxidized form) suspended in 5% gum arabic (verum-group), or only with the latter solvent (placebo-group). Rb(+) can replace up to 60% of the intracellular K(+), and can be used as a tracer of the K(+) movement across the cell membrane. Loading with RbCI was performed from the 3rd week of the idebenone treatment by daily intraperitoneal injections of a dose of 300 mg/kg bw, for 14 days. During the subsequent Rb(+)-release period, the so-called Rb(+)/K(+) discrimination ratio (DR) (Relman et al., 1957, J. Clin. Invest., 36, 1249) was determined on the 3rd and 8th days. Rb(+) and K(+) contents were measured by means of bulk specimen X-ray microanalysis in the intracellular water of brain cells and by atomic absorption spectrophotometry in the serum, in 3-4 animals per group, whereas these concentrations in the cerebrospinal fluid were calculated on the basis of known serum/liquor distribution factors. Normal aging causes a marked increase of DR in brain and liver cells. The values of DR obtained i...Continue Reading

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