The effect of IL-1beta on the expression of inflammatory cytokines and their receptors in human chondrocytes

Life Sciences
Yukiko AidaHideo Matsumura

Abstract

Cytokines released at sites of inflammation and infection can alter the normal processes of cartilage turnover, resulting in pathologic destruction or formation. Interleukin (IL)-1beta plays a central role in the pathophysiology of cartilage damage and degradation in arthritis. In the present study, we examined the effect of IL-1beta on the expression of IL-1beta, IL-6, IL-8, IL-11, tumor necrosis factor-alpha (TNF-alpha), and their receptors in human chondrocytes. The cells were cultured either with or without 100 U/ml of IL-1beta for up to 28 days. The level of expression of the cytokines and their receptors was estimated by determining mRNA levels using real-time PCR or by determining protein levels using ELISA. The expression of IL-1beta, IL-8, and TNF-alpha markedly increased in the presence of IL-1beta after day 14 of culture. The expression of IL-6 and IL-11 increased greatly in the presence of IL-1beta on day 1 and after day 14 of culture. The expression of IL-1beta, IL-8, IL-11, and TNF-alpha receptors significantly decreased in the presence of IL-1beta after day 14 of culture, whereas the expression of IL-6 receptor significantly increased. The expression of these cytokines, except for IL-6, decreased with the additio...Continue Reading

References

Apr 1, 1988·The Journal of Experimental Medicine·P SeckingerJ M Dayer
Dec 30, 1986·Biochemical and Biophysical Research Communications·F OllivierreB V Treadwell
Oct 1, 1995·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·L C DijkgraafR S Liem
Apr 1, 1995·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·K FuW Chen
Aug 1, 1994·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·D M ShaferE F Rossomando
Jan 1, 1997·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·E KubotaK Murakami
Feb 14, 1998·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·E KubotaK I Murakami
Mar 21, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·T TakahashiR Suzuki
May 20, 1998·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·N A SandlerT W Braun
Sep 12, 1998·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·P AlstergrenS Kopp
Aug 7, 1999·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·P K FangK Y Fu
Oct 20, 1999·Frontiers in Bioscience : a Journal and Virtual Library·S R Frenkel, P E Di Cesare
Oct 20, 1999·Frontiers in Bioscience : a Journal and Virtual Library·A R Poole
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L C HofbauerB L Riggs
Jun 5, 2003·Cells, Tissues, Organs·Charles J MalemudTariq M Haqqi
Oct 31, 2003·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Per AlstergrenSigvard Kopp

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Citations

Jan 11, 2007·Journal of Burn Care & Research : Official Publication of the American Burn Association·Timothy P PlackettElizabeth J Kovacs
Aug 10, 2010·BMC Complementary and Alternative Medicine·Karsten GavénisUlrich Schneider
Nov 16, 2007·Arthritis Research & Therapy·Miguel Otero, Mary B Goldring
Feb 20, 2009·Arthritis Research & Therapy·David PretzelRaimund W Kinne
Dec 19, 2013·PloS One·Larissa Cardoso Corrêa AraújoPatrícia Maria Guedes Paiva
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Shun-An LeeShun-Fa Yang
Jun 21, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Xin LvYong-Jin Chen
Sep 30, 2008·Immunological Investigations·Li-Feng HungLing-Jun Ho
Feb 1, 2008·Expert Opinion on Drug Discovery·Gabriel Mbalaviele, Joseph B Monahan
Apr 28, 2007·Veterinary Surgery : VS·Florent DavidSheila Laverty
Dec 17, 2014·BMC Musculoskeletal Disorders·Karl AlvarezRichard O C Oreffo
May 6, 2016·Clinical Orthopaedics and Related Research·Pan TangZhi-Jun Hu
Dec 28, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao LinCuiwu Lin
Apr 10, 2007·Seminars in Fetal & Neonatal Medicine·Adam J WolfbergPierre Gressens
Dec 24, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yong LiangHaiyan Liu
Sep 18, 2020·Biology·Margot NeefjesPeter M van der Kraan
Oct 9, 2019·BMC Complementary and Alternative Medicine·Zhiguang QiaoMing Liu
Jan 18, 2021·European Journal of Pain : EJP·Juliana M TeixeiraJuliana T Clemente-Napimoga
Sep 24, 2020·Bone Research·Eugenie MacfarlaneHong Zhou

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