The effect of in vitro growth conditions on the resistance of Acanthamoeba cysts

The Journal of Eukaryotic Microbiology
Céline CoulonVincent Thomas

Abstract

Despite increasing concerns of direct pathogenicity and/or their role as hosts for other microorganisms there are currently no standard methods for the inactivation of amoebae that belong to the genus Acanthamoeba. Methods used to grow amoebae and produce cysts for these tests may be important as they can dramatically modify cyst susceptibility. We compared resistance of cysts produced from trophozoites grown in peptone-yeast extract-glucose broth or by feeding on HEp-2 cells and then encysted in Neff's medium. We observed that trophozoites grown using HEp-2 cells as a nutrient source produce cysts that are significantly more resistant to SDS and to most biocides tested, including heat. Increased resistance is likely due to a higher proportion of mature cysts presenting thicker cell walls as demonstrated using transmission electron microscopy. This was confirmed by calcofluor white staining demonstrating higher cellulose content in cysts produced from trophozoites grown using HEp-2 cells as a feeding source. These results demonstrate that not only methods used to produce cysts from trophozoites are critical, but that methods used to grow trophozoites before encystment should also be chosen carefully. This should be taken into a...Continue Reading

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Citations

Aug 13, 2013·International Journal of Hygiene and Environmental Health·Mathieu DupuyYann Héchard
Dec 23, 2015·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Nicole Carnt, Fiona Stapleton
Apr 15, 2014·Experimental Parasitology·David Lloyd
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Mar 7, 2021·Pathogens·Monica J CraryManal M Gabriel

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