The effect of inhaled nitric oxide and oxygen on the hydroxylation of salicylate in rat lungs

Pediatric Research
Leif D NelinRalph M Schapira

Abstract

Inhaled nitric oxide (iNO) is used as a selective pulmonary vasodilator, and often under conditions when a high fraction of inspired oxygen is indicated. However, little is known about the potential toxicity of iNO therapy with or without concomitant oxygen therapy. NO can combine with superoxide (O2-) to form peroxynitrite (ONOO-), which can in turn decompose to form hydroxyl radical (OH.). Both OH. and ONOO- are involved in various forms of lung injury. To begin evaluation of the effect of iNO under either normoxic or hyperoxic conditions on OH. and/or ONOO- formation, rats were exposed for 58 h to either 21% O2, 21% O2 + 10 parts per million (ppm) NO, 21% O2 + 100 ppm NO, 50% O2, 90% O2, 90% O2 + 10 ppm NO, or 90% O2 + 100 ppm NO. We used a salicylate hydroxylation assay to detect the effects of these exposures on lung OH. and/or ONOO- formation measured as the appearance of 2,3-dihydroxybenzoic acid (2,3-DHBA). Exposure to 90% O2 and 90% O2 + 100 ppm NO resulted in significantly (p < 0.05) greater lung wet weight (1.99 +/- 0.14 g and 3.14 +/- 0.30 g, respectively) compared with 21% O2 (1.23 +/- 0.01 g). Exposure to 21% O2 + 100 ppm NO led to 2.5 times the control (21% O2 alone) 2,3 DHBA formation (p < 0.05) and exposure to ...Continue Reading

References

Mar 1, 1990·The Journal of Pharmacy and Pharmacology·M J O'Connell, N R Webster
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Jul 1, 1982·Archives of Biochemistry and Biophysics·B A FreemanJ D Crapo
Aug 1, 1995·Archives of Biochemistry and Biophysics·J N LemercierW A Pryor
Feb 1, 1995·American Journal of Respiratory Cell and Molecular Biology·R M SchapiraA D Hacker
May 1, 1994·American Journal of Respiratory Cell and Molecular Biology·R M SchapiraA D Hacker
Jun 1, 1994·The Journal of Pediatrics·S H AbmanJ P Kinsella
Jul 1, 1993·The American Journal of Physiology·Y LuoE Pate
Feb 1, 1993·Circulation·J D RobertsW M Zapol
Jan 1, 1996·Free Radical Biology & Medicine·S GoldsteinG Czapski
Jun 1, 1997·American Journal of Respiratory and Critical Care Medicine·C GaratS Adnot
Jun 11, 1998·Pediatric Research·L D NelinC A Dawson
Jul 8, 1998·Pediatric Clinics of North America·L D Nelin, G M Hoffman
Jul 22, 1998·Chemical Research in Toxicology·G MerényiG Czapski
Aug 15, 1998·Journal of Chemical Neuroanatomy·K P MohanakumarC C Chiueh
Feb 23, 1999·Chemical Research in Toxicology·S GoldsteinG Merényi
Oct 26, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D M GriesK T Nakamura
Oct 29, 2000·Journal of Applied Physiology·P FactorJ I Sznajder
Feb 19, 2002·American Journal of Respiratory and Critical Care Medicine·Phillip FactorVidas Dumasius

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
Hitoshi NishizawaYutaka Takahashi
© 2022 Meta ULC. All rights reserved