The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli

Journal of Biotechnology
Akira ImaizumiKazuhiko Matsui

Abstract

Although the enhancement of amino-acid synthesis by guanosine-3',5'-tetraphosphate (ppGpp) is well known, the effect of intracellular ppGpp levels on amino-acid overproduction in Escherichia coli has not been investigated. In this study, we demonstrate that overexpression of the relA gene, encoding ppGpp synthetase, increases the accumulation of amino acids, such as glutamate and lysine, in amino-acid-overproducing strains of E. coli. Elevation of intracellular ppGpp levels due to depletion of required amino acids also enhances glutamate overproduction. Moreover, the extent of overproduction is highly dependent on the intracellular ppGpp level. These results demonstrate that amino-acid overproduction in E. coli is closely connected to amino-acid auxotrophy via the accumulation of ppGpp.

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Citations

Jun 14, 2015·Metabolic Engineering·Jingyu Wang, Kechun Zhang
Feb 27, 2013·Journal of Microbiological Methods·Giovanni LongoSandor Kasas
Jun 22, 2018·Applied Microbiology and Biotechnology·Jingyu WangKechun Zhang
May 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Madeline E SherlockRonald R Breaker

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