PMID: 6412701Aug 15, 1983Paper

The effect of ionophore A23187 on calcium ion fluxes and alpha-adrenergic-agonist action in perfused rat liver

The Biochemical Journal
P H ReinhartF L Bygrave

Abstract

The effect of ionophore A23187 on cellular Ca2+ fluxes, glycogenolysis and respiration was examined in perfused liver. At low extracellular Ca2+ concentrations (less than 4 microM), A23187 induced the mobilization of intracellular Ca2+ and stimulated the rate of glycogenolysis and respiration. As the extracellular Ca2+ concentration was elevated, biphasic cellular Ca2+ fluxes were observed, with Ca2+ uptake preceding Ca2+ efflux. Under these conditions, both the glycogenolytic response and the respiratory response also became biphasic, allowing the differentiation between the effects of extracellular and intracellular Ca2+. Under all conditions examined the rate of Ca2+ efflux induced by A23187 was much slower than the rate of phenylephrine-induced Ca2+ efflux, although the net amounts of Ca2+ effluxed were similar for both agents. The effect of A23187 on phenylephrine-induced Ca2+ fluxes, glycogenolysis and respiration is dependent on the extracellular Ca2+ concentration. At concentrations of less than 50 microM-Ca2+, A23187 only partially inhibited alpha-agonist action, whereas at 1.3 mM-Ca2+ almost total inhibition was observed. The action of A23187 at the cellular level is complex, dependent on the experimental conditions u...Continue Reading

Citations

Nov 1, 1988·Biological Reviews of the Cambridge Philosophical Society·J G Altin, F L Bygrave
Nov 1, 1984·Biological Reviews of the Cambridge Philosophical Society·P H ReinhartF L Bygrave
Feb 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ming FuRui Wang
Aug 3, 2004·Microscopy Research and Technique·Othon B KotoulasDimitrios J Kondomerkos
Feb 17, 2001·General and Comparative Endocrinology·G KrumschnabelP J Schwarzbaum

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