The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

BioMed Research International
Agnieszka PartykaWojciech Niżański

Abstract

The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P< 0.01 andP< 0.05) following cryopreservation. After thawing, we observed a higher percentage of sperm without apoptosis and membrane reorganization changes in the LC1 and T1 group when compared to the control (P< 0.05). There was a higher percentage of live sperm without lipid peroxidation (LPO) in all treatments (P< 0.01;P< 0.05), when compared to the control group. The percentage of sperm with high mitochondrial potential significantly increased with LC1, T1, and T10 (P< 0.05). Supplementation of the diluent with LC1, LC5, and T1 significantly (P< 0.05) reduced DNA susceptibility to fragmentation, compared to the control and HT1 groups. These results indicate that the addition of examined an...Continue Reading

References

Nov 1, 1978·Poultry Science·N Fujihara, B Howarth
Jan 1, 1992·Physiological Reviews·R J Huxtable
Jan 1, 1987·Advances in Experimental Medicine and Biology·J H FellmanA L Eicher
Jan 1, 1995·Reproduction, Fertility, and Development·R J Aitken
Dec 1, 1995·British Poultry Science·K TselutinB Tur
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P F SuraiN H Sparks
Feb 5, 2000·Molecular Reproduction and Development·J F BilodeauC Gagnon
May 4, 2002·Animal Reproduction Science·Robert H FooteMichael T Kaproth
Sep 23, 2003·Molecular Reproduction and Development·Christelle BréqueJean-Pierre Brillard
Feb 20, 2004·Biology of Reproduction·Guillaume MartinRachel Levy
Dec 14, 2004·Annals of the New York Academy of Sciences·Chiu Ming NgRonald S Swerdloff
Nov 6, 2007·Theriogenology·E BlesboisM M Mialon-Richard
Jun 29, 2010·Theriogenology·Agnieszka PartykaEwa Łukaszewicz
Jun 15, 2011·Theriogenology·Agnieszka PartykaWojciech Niżański
Aug 14, 2012·Animal Reproduction Science·Agnieszka PartykaWojciech Niżański
Jun 19, 2013·Cryobiology·Agnieszka PartykaMałgorzata Ochota
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·S Manee-InK Kaeoket
Jun 18, 2015·Zygote : the Biology of Gametes and Early Embryos·Wei ZhangWenqin Lin
Oct 23, 2016·Theriogenology·Valentina LongobardiBianca Gasparrini

❮ Previous
Next ❯

Citations

Dec 23, 2017·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yu-Jie ZouWen Long
Oct 3, 2018·Biological & Pharmaceutical Bulletin·Tomohiro NishimuraEmi Nakashima
Nov 27, 2018·Reproduction in Domestic Animals = Zuchthygiene·Martyna PolitWojciech Niżański
Jan 30, 2019·Andrologia·Gui-Chao DaiJian-Hong Hu
Jun 9, 2020·Reproduction in Domestic Animals = Zuchthygiene·Artur KowalczykJoanna Rosenberger
May 27, 2020·Molecular Reproduction and Development·Kaustubh K SarafAyyasamy Manimaran
Aug 14, 2019·Andrologia·Leila NajafiMansoureh Movahedin
Jan 21, 2020·Reproductive Medicine and Biology·Kazutaka TeraiAkira Tsujimura
Aug 14, 2020·International Journal of Molecular Sciences·Julian Santiago-Moreno, Elisabeth Blesbois
Mar 7, 2021·Andrology·Alicja Kowalczyk, Ewa Czerniawska Piątkowska

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
MDA

Software Mentioned

[UNK]
STATISTICA

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis