The effect of leptin is enhanced by microinjection into the ventromedial hypothalamus

Diabetes
R J JacobR S Sherwin

Abstract

To determine whether changes in food intake produced by leptin involve targeting the hormone to distinct central nervous system regions, guide cannulas were positioned stereotaxically into three brain regions--the ventromedial hypothalamus (VMH) (bilaterally, n = 6), the dorsal raphe nucleus (n = 3), and the lateral ventricle (n = 3)--of nonobese male rats (400-500 g). Daily food intake and body weight changes were measured during twice-daily injections of saline (0.1 microl) followed by recombinant human leptin (0.05 microg) for 3 days via the brain cannulas. VMH-injected rats also were followed during a postleptin saline recovery interval. This small dose of leptin did not change food intake or body weight from that during the preceding saline injection period in ventricle-injected or dorsal raphe-injected rats. In sharp contrast, VMH-injected rats ate much less food (56 +/- 8% basal) and lost 9 +/- 3 g/day or 5% of their body weight during 3 days of leptin administration. VMH-injected animals fully recovered from leptin-induced effects within 3 days. We conclude that small doses of leptin that do not effect eating behavior when delivered to the ventricle or the dorsal raphe (another brain region believed to regulate feeding)...Continue Reading

Citations

Nov 19, 2002·Journal of Neurobiology·Young-Hwan JoLorna W Role
May 4, 2000·Domestic Animal Endocrinology·S DridiM Taouis
May 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D RothAlain D Baron
Apr 20, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·Theodore P Braun, Daniel L Marks
Dec 6, 2000·The Journal of Small Animal Practice·R W Nelson
May 18, 2007·Annual Review of Neuroscience·Qian Gao, Tamas L Horvath
Aug 15, 2000·Annual Review of Nutrition·C A BaileR J Martin
Jun 3, 2011·The Journal of Clinical Investigation·Laurent Gautron, Joel K Elmquist
Jun 17, 2008·Endocrinology·Boman G IraniBarry E Levin
Mar 4, 2005·Treatments in Endocrinology·Kim S Bell-Anderson, Janet M Bryson
Dec 7, 2007·FEBS Letters·Qian Gao, Tamas L Horvath
Jun 14, 2006·Developmental Psychobiology·Gerard P Smith
Nov 2, 2006·Journal of Neurochemistry·Jenni Harvey
Aug 24, 1999·Annals of Internal Medicine·R A DeFronzo
Feb 14, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James W Maniscalco, Linda Rinaman
Aug 2, 2014·The Journal of Clinical Investigation·Franck ChiappiniBarbara B Kahn
Apr 2, 2020·American Journal of Physiology. Endocrinology and Metabolism·Ruth B S Harris
Jul 24, 2004·Endocrinology·Heike MünzbergChristian Bjørbaek
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Claire A MatsonRobert C Ritter
May 2, 1998·Nutrition Reviews·J M Friedman
Nov 13, 1998·The Journal of Biological Chemistry·L LiuL Rossetti
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·A SugaS Inoue
Feb 26, 2000·Diabetic Medicine : a Journal of the British Diabetic Association·B H WolffenbuttelR N Patwardhan
Aug 12, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·V A Boundy, A H Cincotta
May 22, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·Y Liang, A H Cincotta
Nov 12, 2003·Experimental Biology and Medicine·Hiroyoshi UtsumiKazuma Fujimoto
Nov 12, 2016·The Biochemical Journal·Ricardo LageMiguel López
Aug 5, 1998·The American Journal of Physiology·M C FlynnC R Plata-Salamán
Jun 8, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·ChuanFeng WangCatherine M Kotz
Jul 23, 2008·Developmental Disabilities Research Reviews·Pauline M Smith, Alastair V Ferguson
Oct 6, 1997·Medicinal Research Reviews·X RemesarM Alemany

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.