The effect of leptin on Na(+)-H(+) antiport (NHE 1) activity of obese and normal subjects erythrocytes

Molecular and Cellular Endocrinology
A Konstantinou-TegouG Koliakos

Abstract

Obesity is currently considered as a chronic metabolic disease, associated with a high risk of cardiovascular complications. Leptin, an adipocyte-derived hormone has a variety target cells influencing a wide range of processes. Possible counteractions of hyperleptinaemia are currently investigated. The Na(+)-H(+) exchanger (NHE 1) is involved in multiple cellular functions and its activation has been related to hypertension and obesity. NHE 1 is present on erythrocytes and can be stimulated by various hormones. Erythrocytes have on their surface a variety of receptors with mostly unknown function. In the present paper, the effect of leptin on erythrocytes NHE 1 activity has been investigated. For this reason, the intracellular pH and sodium influxes were measured before and after addition of leptin in erythrocyte suspensions from normal and obese individuals. Amiloride, a specific NHE 1 inhibitor, and staurosporine a protein kinase C inhibitor were used to inhibit erythrocyte NHE 1. For the binding study leptin was labeled with fluorescein isothiocyanate (FITC) and the binding on erythrocytes was estimated by Scatchard analysis. NHE 1 activity increased in the presence of leptin but significantly less in the obese than in the c...Continue Reading

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Citations

Mar 21, 2009·Cell Stress & Chaperones·Diamantis KonstantinidisMartha Kaloyianni
Aug 2, 2003·Molecular and Cellular Endocrinology·Dimitris BourikasGeorge Koliakos
Apr 14, 2006·Journal of Hypertension·Jerzy Bełtowski
Aug 13, 2010·Diabetes Research and Clinical Practice·D Simmons
Aug 2, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Martha KaloyianniStefanos Dailianis
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Mar 2, 2019·Expert Opinion on Drug Metabolism & Toxicology·Alvaro Garcia-RoperoJuan J Badimon
Dec 17, 2010·The Open Cardiovascular Medicine Journal·Maria SarigianniKonstantinos Paletas

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