The effect of lipopolysaccharide on the expression level of immunomodulatory and immunostimulatory factors of human amniotic epithelial cells

BMC Research Notes
Ramezan Ali TaheriMahmood Saffari

Abstract

Human amniotic epithelial cells (hAECs) are a novel source of stem cells and have immunomodulatory effects on both the innate and adoptive immune system. hAECs can differentiate into multiple cell lineages that make them a suitable cell source for regenerative medicine. These cells express multiple toll-like receptors (TLRs) and respond to various TLR ligands. This study aimed to evaluate the effect of lipopolysaccharide (LPS), a TLR4 ligand, on the level of immunomodulatory and immunostimulatory factors of hAECs. Our results indicated that LPS had the ability to up-regulate the expression of prostaglandin E2 synthase and transforming growth factor-beta1 in hAECs. However, there was no change in the level of interleukin-1beta, interleukin-6 and interleukin-10 in hAECs when were stimulated with LPS. In addition, we observed tumor necrosis factor-alpha was only expressed at very low level in some of hAECs samples which its expression was independent of the effects of LPS.

References

Dec 28, 1999·Microbes and Infection·D A Lebman, J S Edmiston
Feb 25, 2005·Investigative Ophthalmology & Visual Science·Haochuan LiHassan Alizadeh
Jul 5, 2005·Placenta·V M Abrahams, G Mor
Aug 6, 2005·Stem Cells·Toshio MikiStephen C Strom
Jul 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claire GillauxMichelle Breuiller-Fouché
Oct 4, 2012·Cell Transplantation·Antonia AlcarazFrancisco J Nicolás

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Citations

Jul 7, 2020·Frontiers in Physiology·Justin G PadronClaire E Kendal-Wright
Jan 10, 2021·Scientific Reports·Hamid KhodayariAli Mohammad Alizadeh

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

Biosystems
REST
Relative Expression Software Tool
Applied

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