The effect of low-level laser irradiation on sperm motility, and integrity of the plasma membrane and acrosome in cryopreserved bovine sperm

PloS One
Guilherme Henrique C FernandesRegiane Albertini

Abstract

Freezing changes sperm integrity remarkably. Cryopreservation involves cooling, freezing, and thawing and all these contribute to structural damage in sperm, resulting in reduced fertility potential. Low-level laser irradiation (LLLI) could increase energy supply to the cell and cause reactive oxygen species reduction (ROS), contributing to the restoration of oxygen consumption and adenosine triphosphate synthesis (ATP) in the mitochondria. Our goal was to analyze the effects of low-level laser irradiation on sperm motility and integrity of the plasma membrane and acrosome in cryopreserved bovine sperm. We analyzed 09 samples of bull semen (Bos taurus indicus), divided into three groups: a control group without laser irradiation, a 4J group subjected to a laser irradiation dose of 4 joules, and a 6J group subjected to dose of 6 joules. Samples were divided for the analysis of cell viability and acrosomal membrane integrity using flow cytometry; another portion was used for motion analysis. Irradiation was performed in petri dishes of 30 mm containing 3 ml of semen by an aluminum gallium indium phosphide laser diode with a wavelength of 660 nm, 30 mW power, and energy of 4 and 6 joules for 80 and 120 seconds respectively. Subseq...Continue Reading

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Citations

Jun 14, 2015·Tropical Animal Health and Production·Manoel Lopes Silva FilhoMarcos Antonio Lemos de Oliveira
Jun 9, 2016·Lasers in Medical Science·Adriano F P SiqueiraMayra E O A Assumpção
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Apr 2, 2021·Lasers in Surgery and Medicine·Burkhard T EspeyKatrin van der Ven

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Methods Mentioned

BETA
semen collection
flow cytometry
Fluorescence

Software Mentioned

GraphPad
Integrated Semen Analysis System
ISAS Integrated Semen Analysis System
GraphPad Prism

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