PMID: 11896938Mar 19, 2002

The effect of LPS on neutrophils from patients with high risk of type 1 diabetes mellitus in relation to IL-8, IL-10 and IL-12 production and apoptosis in vitro

Scandinavian Journal of Immunology
Ewa GlowackaH Tchórzewski

Abstract

Innate immunity includes neutrophil inflammatory function, tissue destruction and regulatory cytokine production. Programmed cell death (apoptosis) is postulated to be a key mechanism for neutrophil elimination during inflammation. The aim of the present study was to evaluate the neutrophil apoptosis in relation to IL-8, IL-10 and IL-12 production in vitro by neutrophils of patients suffering from diabetes mellitus (DM)1 and the first-degree relatives of patients with DM1. The early stage of neutrophils apoptosis was assessed morphologically, and the later stage by DNA-binding dye propidium iodide, both after treatment with lipopolysaccharide (LPS), insulin or anti-CD95 antibody (Ab) as stimulators. CD16 (FcgammaRIII) receptor expression was also evaluated. Production of IL-8, IL-10, and IL-12 cytokine was evaluated in supernatant after neutrophil incubation for 21 h in culture medium alone, in medium in the presence of LPS, insulin or anti-CD95 antibody (Ab). Cytokine concentrations were measured by enzyme-linked immunosorbent assay (ELISA) method using commercially available kits. Our study demonstrates that LPS inhibits the early stage of apoptosis (as evaluated morphologically) of healthy donors' neutrophils. The LPS-depend...Continue Reading

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Citations

Aug 21, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Tsan-Tzu YangChiou-Feng Lin
Apr 11, 2009·Apoptosis : an International Journal on Programmed Cell Death·Aidan RyanFionnuala B Hickey
Jul 15, 2003·Mediators of Inflammation·Krzysztof SzylloAnna Kamer-Bartosinska
Dec 5, 2013·International Journal of Evidence-based Healthcare·Brian CheeP Mark Bartold
Oct 11, 2007·Diabetes, Obesity & Metabolism·Sayeeda RahmanAbdul Rahman A Rashid
Jul 21, 2007·Mediators of Inflammation·K ZorenaK Raczyńska
Apr 11, 2009·Journal of Immunological Methods·Christian SmedmanStaffan Paulie
Nov 15, 2006·Clinical and Experimental Immunology·Elaine HatanakaAna Campa
Aug 17, 2006·Shock·Yoram VodovotzGilles Clermont
Jul 1, 2005·Shock·Carson C ChowYoram Vodovotz
Jun 18, 2017·Journal of the American Society of Nephrology : JASN·Dawn J CasterK R McLeish
Mar 31, 2006·Diabetes/metabolism Research and Reviews·M R GoodierM Londei
Dec 13, 2019·Mediators of Inflammation·Leandro BorgesElaine Hatanaka
Sep 1, 2005·Molecular and Cellular Biochemistry·Cacchio MarisaM Reale

Related Concepts

Autoantigens
Diabetes, Autoimmune
Novolin
Lipopolysaccharides
Neutrophil Band Cells
Prediabetes Syndrome
Nested Case-Control Studies
Interleukin-8
Interleukin-10
Apoptosis, Intrinsic Pathway

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