The effect of magnolol on Ca2+ homeostasis and its related physiology in human oral cancer cells

Archives of Oral Biology
Shu-Feng HsiehChung-Ren Jan

Abstract

Magnolol, a polyphenol compound from herbal medicines, was shown to alter physiology in various cell models. However, the effect of magnolol on Ca2+homeostasis and its related physiology in oral cancer cells is unclear. This study examined whether magnolol altered Ca2+signaling and cell viability in OC2 human oral cancer cells. Cytosolic Ca2+concentrations ([Ca2+]i) in suspended cells were measured by using the fluorescent Ca2+-sensitive dye fura-2. Cell viability was examined by 4-[3-[4-lodophenyl]-2-4(4-nitrophenyl)-2H-5-tetrazolio-1,3-benzene disulfonate] water soluble tetrazolium-1 (WST-1) assay. Magnolol at concentrations of 20-100 μM induced [Ca2+]irises. Ca2+removal reduced the signal by approximately 50%. Magnolol (100 μM) induced Mn2+influx suggesting of Ca2+entry. Magnolol-induced Ca2+entry was partially suppressed by protein kinase C (PKC) regulators, and inhibitors of store-operated Ca2+channels. In Ca2+-free medium, treatment with the endoplasmic reticulum Ca2+pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) abolished magnolol-evoked [Ca2+]irises. Conversely, treatment with magnolol abolished BHQ-evoked [Ca2+]irises. Inhibition of phospholipase C (PLC) with U73122 partially inhibited magnolol-induced [Ca2+]irises...Continue Reading

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Citations

Aug 15, 2018·International Journal of Molecular Sciences·Abhishek Manoj RanawareAjaikumar B Kunnumakkara
Jun 27, 2019·BioMed Research International·Jianhong ZhangLonghuo Wu
Jan 22, 2021·Environmental Science and Pollution Research International·Haiying WeiHong Geng
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zile HuangJiangang Wang
Apr 6, 2021·Frontiers in Pharmacology·Yiping LinYing Song

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