The effect of matrix extracellular phosphoglycoprotein and its downstream osteogenesis-related gene expression on the proliferation and differentiation of human dental pulp cells

Journal of Endodontics
Xi WeiJunqi Ling

Abstract

Matrix extracellular phosphoglycoprotein (MEPE), a new member of the small integrin binding ligand N-glycosylated (SIBLING) family, is believed to play multifunctional roles in regulation of cell signaling, mineral homeostasis, and mineralization. To study how MEPE affects the downstream genes involved in regulation of the proliferation and osteogenesis differentiation of dental pulp cells (DPCs), we explored the proliferation and osteogenesis differentiation capability of DPCs stimulated with recombinant MEPE or transfected with adenoviral-mediated human MEPE gene and used a systematic approach by osteogenesis real-time polymerase chain reaction arrays to profile osteogenesis-related gene expression after MEPE gene transfection. Our results indicated higher proliferation capability in a time- and dose-dependent pattern by cholecystokinin octapeptide assay, and gene/protein expression of osteogenic markers bone sialoprotein, dentin sialophosphoprotein, osteocalcin, and collagen I were up-regulated dependent on time points showed by real-time polymerase chain reaction and Western blot. Moreover, a total of 3 genes, including enamelin, transforming growth factor-β2, and integrin α2, were significantly up-regulated. These results ...Continue Reading

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Citations

Nov 5, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chia-Yung LinMin-Huey Chen
Mar 6, 2013·Journal of Endodontics·Kenneth M HargreavesFabricio B Teixeira
Jul 21, 2012·European Journal of Oral Sciences·Michal JágrIvan Mikšík
Mar 2, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Angela GullardMary MacDougall
May 13, 2021·Clinical Oral Investigations·Yaghoup Feridouni KhamanehReinhard E Friedrich
Jul 27, 2021·Tissue Engineering. Part B, Reviews·Satnam Singh VirdeePhillip L Tomson

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