The Effect of Mesenchymal Stem Cell-Derived Microvesicles on Erythroid Differentiation of Umbilical Cord Blood-Derived CD34+ Cells

Advanced Pharmaceutical Bulletin
Davod Pashoutan SarvarKarim Shamsasenjan

Abstract

Purpose: Mesenchymal stem cells (MSCs) play an important role in the proliferation and differentiation of hematopoietic stem cells (HSCs) in the bone marrow via cell-to-cell contact, as well as secretion of cytokines and microvesicles (MVs). In this study, we investigated the effect of mesenchymal stem cell-derived microvesicles (MSC-MVs) on erythroid differentiation of umbilical cord blood-derived CD34+ cells. Methods: In this descriptive study, CD34+ cells were cultured with mixture of SCF (10 ng/ml) and rhEPO (5 U/ml) cytokines in complete IMDM medium as positive control group. Then, in MV1- and MV2-groups, microvesicles at 10 and 20 µg/ml concentration were added. After 72 hours, erythroid specific markers (CD71 and CD235a) and genes (HBG1, GATA1, FOG1 and NFE2) were assessed by flow cytometry and qRT-PCR, respectively. Results: The expression of specific markers of the erythroid lineages (CD71 and GPA) in the presence of different concentration of microvesicles were lower than that of the control group (P<0.001). Also, the expression of specific genes of the erythroid lineages (NFE2, FOG1, GATA1, and HBG1) was investigated in comparison to the internal control (GAPDH). Among all of them, HBG1 and FOG1 genes were signi...Continue Reading

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Citations

Jul 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhuo LiangYonggang Lv
Jul 20, 2020·Ageing Research Reviews·Behnaz Banimohamad-ShotorbaniMohammad Reza Sadeghi
Jun 4, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Laura R Goldberg

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Methods Mentioned

BETA
flow cytometry
Dynamic Light Scattering

Software Mentioned

GraphPad Prism
CellQuest
Flowing
GraphPad
FACSCalibur

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