The effect of metals on SDS-induced partially folded states of CopC

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Zhen SongBinsheng Yang

Abstract

In this work, the unfolding of CopC was used to elucidate details of the protein structure through different spectroscopic techniques. The interactions of CopC and its mutants with the anionic surfactant sodium dodecyl sulfate (SDS), guanidinium hydrochloride, and urea were monitored by fluorescence spectroscopy, far-UV circular dichroism spectroscopy, and fluorescence lifetime measurements. The interaction of SDS with CopC resulted in the formation of a partially folded intermediate. In this intermediate, the structure of the C-terminal is unfolded, whereas the N-terminal retains the native structure. Further, we have explored the effects of metals on the intermediate in aqueous surfactant. The results suggested that the Ag(+) ion has a large effect on the unfolding induced by SDS. In addition, the binding capacity of the different unfolding degree protein toward Cu(2+) indicated the high stability of the N-terminal. The protein-Cu(2+) unfolding induced by guanidinium hydrochloride and urea caused the binding of Cu(2+) to increase the stability of the N-terminal, which resulted in an intermediate in the unfolding process. The first transition corresponded to unfolding of the C-terminal, and the second transition was attributed...Continue Reading

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Citations

Jan 9, 2016·Biophysical Journal·R Bryn Fenwick, H Jane Dyson
Jun 17, 2016·Journal of Photochemistry and Photobiology. B, Biology·Zhen SongBinsheng Yang

Related Concepts

CopC protein, Bacteria
Bacterial Proteins
Metals
Irium
Point Mutation
Protein Conformation, beta-Strand
Protein Folding, Globular
Protein Unfolding
Circular Dichroism
Copper

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