The Effect of Microfluidic Geometry on Myoblast Migration

Micromachines
Rahul AtmaramaniNesreen Zoghoul Alsmadi

Abstract

In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels-from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5⁻20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration.

References

Jun 10, 2000·Molecular and Cellular Biology·J SuzukiH Koide
Jul 18, 2000·Experimental Cell Research·E El FahimeJ P Tremblay
Jul 20, 2001·Experimental Cell Research·S CortiG Scarlato
Dec 6, 2002·American Journal of Physiology. Cell Physiology·David L AllenKotoku Kurachi
Sep 9, 2005·Nature Reviews. Cancer·Thomas BrabletzThomas Kirchner
Oct 6, 2005·Trends in Cell Biology·Paul Martin, S Joseph Leibovich
Dec 22, 2005·Nature Immunology·Andrew D LusterUlrich H von Andrian
May 23, 2008·Molecular Biology of the Cell·Christopher BeadlePeter Canoll
Nov 13, 2008·Journal of Cell Science·Magali LouisPhilippe Gailly
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Daniel Irimia, Mehmet Toner
Nov 27, 2010·Current Opinion in Cell Biology·Peter FriedlJan Lammerding
Dec 15, 2010·Analytical Biochemistry·K P Goetsch, C U Niesler
Jan 27, 2012·PloS One·Ziqiu TongKonstantinos Konstantopoulos
Jun 5, 2012·Annual Review of Biomedical Engineering·Rui P MartinsDavid A Lee
Aug 7, 2012·Lab on a Chip·Yi FuAntonius M J VanDongen
Jun 19, 2013·Journal of Neuroscience Methods·Katherine A SouthamTracey C Dickson
Aug 24, 2013·Lab on a Chip·Chukwuemeka George Anene-NzeluHanry Yu
Aug 28, 2013·The Journal of Cell Biology·Wei-Chien HungKonstantinos Konstantopoulos
Oct 11, 2013·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Hyun Sung ParkIn Hong Yang
Dec 11, 2013·Tissue Engineering. Part B, Reviews·Serge OstrovidovAli Khademhosseini
Apr 25, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Meghaan M FerreiraSarah C Heilshorn
Jul 16, 2016·Annual Review of Biomedical Engineering·Colin D PaulKonstantinos Konstantopoulos
Oct 17, 2017·Frontiers in Cellular Neuroscience·Bryan J BlackJoseph J Pancrazio
Jun 12, 2017·ACS Biomaterials Science & Engineering·Sharmistha NaskarBikramjit Basu

❮ Previous
Next ❯

Methods Mentioned

BETA
chips
fluorescence microscopy
fluorescence imaging

Software Mentioned

Origin Pro
OriginPro
ImageJ
OriginLab

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.