The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study

Journal of Endodontics
Yoshiyuki YasudaTakashi Saito

Abstract

The aim of this study was to investigate the effect of mineral trioxide aggregate (MTA) on cell viability and mineralization ability of rat dental pulp cells. The pulp capping materials, such as MTA, Dycal (Dentsply Caulk, Milford, DE), and Superbond C&B (SB; Sun Medical, Shiga, Japan) were placed on transwell inserts and cultured with rat dental pulp cells. MTA and SB exhibited no cytotoxicity, whereas almost all cells had died after 72 hours of culture with Dycal. MTA significantly stimulated mineralization by 60% compared with the control. MTA and Dycal significantly upregulated by two-fold the level of bone morphogenetic protein (BMP)-2 messenger RNA expression compared with the control. Furthermore, MTA increased BMP-2 protein production by about 40%, whereas Dycal significantly reduced it. Although MTA and Dycal increased the concentration of extracellular calcium by about 0.4 mmol/L, SB had no effect. These results suggest that BMP-2 may play an important role in mineralization stimulated by MTA.

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May 24, 2008·Journal of Endodontics·Yoshiyuki YasudaTakashi Saito

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Citations

Apr 12, 2014·ISRN Dentistry·Aniket S WadajkarTakashi Komabayashi
Feb 14, 2013·Journal of Endodontics·Young-Hee LeeHo-Keun Yi
Jun 19, 2012·Journal of Endodontics·Jong-Jin KimEun-Cheol Kim
Mar 1, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·B W Darvell, R C T Wu
Sep 1, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Kyung-San MinEun-Cheol Kim
Jun 30, 2009·Acta histochemica·Yoshiko Masuda-MurakamiKoukichi Matsumoto
Mar 22, 2014·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Nawaf LabbanJeffrey A Platt
Jan 19, 2012·Acta Odontologica Scandinavica·Mohammad Ali SaghiriAhmad Sheibaninia
Jan 18, 2011·Journal of Endodontics·Ana Lívia Gomes CornélioMário Tanomaru Filho
Aug 16, 2016·International Endodontic Journal·L M S Castro-RaucciP T de Oliveira
Dec 30, 2016·Journal of Dental Research·S PhungR H Kim
Feb 9, 2017·International Endodontic Journal·M Collado-GonzálezF J Rodríguez-Lozano
Aug 8, 2014·Journal of Biomaterials Applications·Ga-Hyun KimEun-Cheol Kim
Dec 6, 2017·Materials·Bill KahlerLouis M Lin
Feb 15, 2018·European Journal of Oral Sciences·Liliana Argueta-FigueroaMa C Arenas-Arrocena
Mar 27, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kaori HanadaChiaki Kitamura
Oct 3, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hae Won ChoiSang-Hoon Rhee
Oct 18, 2018·Brazilian Oral Research·Marcela Carvalho EspaladoriAntônio Paulino Ribeiro Sobrinho
May 21, 2019·Dental Materials Journal·Panruethai TrongkijDanuchit Banomyong

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