The effect of monosaccharide sugars and pyruvate on the differentiation and metabolism of sheep granulosa cells in vitro.

Reproduction : the Official Journal of the Society for the Study of Fertility
B K CampbellR J Scaramuzzi

Abstract

The objective of this study was to investigate the effect of three monosaccharides or pyruvate on the ability of gonadotrophins to induce cellular proliferation and differentiation of cultured sheep granulosa cells. Lactate production and levels of mRNA expression for the glucose transporters SLC2A1, SLC2A4, SLC2A5 and SLC2A8 were also determined. No energy source in the culture media reduced cell number (50%) and oestradiol (E(2)) production. Dose and type of monosaccharide had a highly significant (P<0.001) effect on FSH-induced differentiation of the granulosa cells, and there was a highly significant interaction (P<0.001). Glucose supported higher levels of E(2) production than fructose, which was in turn higher than galactose (P<0.001). In contrast, pyruvate at low doses supported similar levels of E(2) production as glucose, but higher doses were markedly inhibitory to E(2) production (P<0.001). Cells responded positively to insulin (P<0.001) in the presence of all three monosaccharides. Glucose and the high doses of fructose resulted in the accumulation of lactate (P<0.001), but pyruvate, galactose and the low dose of fructose resulted in low lactate production. SLC2A5 expression was not detected and SLC2A8 expression wa...Continue Reading

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Citations

Aug 19, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·B K CampbellR J Scaramuzzi
Feb 14, 2018·Journal of Molecular Endocrinology·Zhengjie YanYugui Cui
Apr 20, 2017·Reproduction, Fertility, and Development·David A LandryMarc-André Sirard
Mar 11, 2020·Zygote : the Biology of Gametes and Early Embryos·Ahmed S A SosaMahmoud F Nawito
Nov 20, 2016·Annals of the New York Academy of Sciences·Elissa Z CameronLaura M Parsley
May 29, 2016·The Biochemical Journal·Joëlle Dupont, Rex J Scaramuzzi
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Edward N O'NeillDavid E Block
Feb 8, 2021·Biology of Reproduction·Abigail M Maucieri, David H Townson
Jul 6, 2021·Molecular Reproduction and Development·Kahina Ghanem, Alan L Johnson

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