Apr 23, 1982

The effect of monovalent cations on calcium efflux in yeasts

Biochimica Et Biophysica Acta
Y Eilam

Abstract

The properties of the calcium efflux system in the yeast Saccharomyces cerevisiae were investigated. After growing the cells overnight in medium containing 45Ca, the cells were transferred to medium containing glucose. Herpes buffer (pH 5.2) and monovalent cations. The presence of potassium or sodium in the medium induced efflux of calcium from the cells. The magnitude of the efflux was dependent on the concentration of these cations in the medium. The time course of calcium efflux was analyzed, and two types of exchangeable calcium pools, which turned over at different rates, were detected: 'Fast turnover' and 'slow turnover'. Increase in the concentration of monovalent cations in the medium caused an increase in the fraction of cellular calcium which turned over at a fast rate, and activation of calcium efflux from the 'slow turnover' calcium pool. The specific changes in the parameters of calcium efflux induced by monovalent cations were different from those reported previously to be induced by divalent cations. Both processes, i.e. activation of calcium efflux by monovalent and by divalent cations, were found to be additive, indicating that they operate via different mechanisms. Experiments using the respiratory inhibitor A...Continue Reading

Mentioned in this Paper

Cations, Divalent
Calcium [EPC]
Herpes NOS
Calcium
Saccharomyces cerevisiae allergenic extract
Antimycin A
Yeasts
Cations
Fas-activated Serine/Threonine Kinase Activity
Biochemical Turnover

About this Paper

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