The effect of mouse lung granulocyte-macrophage colony-stimulating factor and other colony-stimulating activities on the proliferation and differentiation of murine bone marrow cells in long-term cultures

Journal of Cellular Physiology
N Williams, A W Burgess

Abstract

The roles of colony-stimulating factors in long-term bone marrow cultures were studied and compared. After single additions of high concentrations of unpurified colony-stimulating activities to the cultures, rapid deterioration of the cultures was observed. This appears to result from contaminants in the stimulatory preparations. Cultures to which one purified colony-stimulating factor (csf) from endotoxin mouse lung-conditioned medium was added did not run down ten weeks after addition and were found to be the same as the controls. The deterioration of the cultures to which unpurified stimulators were added could not be accounted for by accelerated granulopoiesis leading to subsequent exhaustion of the cultures. The inability of purified CSF to affect the cellularity of the suspension cells did not result from instability or masking of the activity in the cultures, nor did CSF preferentially stimulate the cells within the adherent layer. The suspension cells responded to purified CSF after separation from the adherent cells. The data suggest that if CSFs are marrow stimulators, their effects in turn may be stringently regulated within the marrow.

References

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Citations

Apr 15, 1985·Klinische Wochenschrift·H G Mergenthaler
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·T M Dexter
Jan 1, 1987·Comparative Immunology, Microbiology and Infectious Diseases·J G BenderC C Stewart
Aug 15, 1987·Cancer·J L AscensaoM E Kaplan
Jan 1, 1982·Journal of Cellular Physiology. Supplement·T M Dexter
Jan 1, 1983·Journal of Cellular Physiology·R K ShadduckT M Dexter
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·D Zipori
Nov 5, 2019·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Giovanni DamianiMahmoud A Ghannoum
Dec 1, 1987·Immunology Letters·J M Garland
Jan 1, 1988·Critical Reviews in Oncology/hematology·H E Broxmeyer, D E Williams

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