The effect of mucoadhesive excipient on the nasal retention time of and the antibody responses induced by an intranasal influenza vaccine

Vaccine
Shinji SaitoHideki Hasegawa

Abstract

Recently, we reported that intranasal vaccination of humans with whole inactivated influenza vaccine in the absence of mucosal adjuvant induced neutralizing antibody responses in the serum and nasal mucus. The mucoadhesive excipient carboxy-vinyl polymer (CVP) increases the viscosity and therefore mucoadhesiveness of intranasal medicaments and is an authorized excipient in Japan. In the present study, we analyzed the effect of adding CVP on intranasal whole inactivated influenza vaccine antigen dynamics and antibody responses. Mice and nonhuman primates (NHPs) were intranasally administered the [(18)F]-radiolabeled vaccine and subjected to positron emission tomography analysis for 6h. Dendritic cells were stimulated in vitro with the vaccine mixed with or without a mucosal adjuvant (Ampligen) and/or CVP, after which the tumor necrosis factor (TNF)-α and interferon (IFN)-β levels in the supernatants were measured. Cynomolgus monkeys were immunized intranasally with the vaccine mixed with Ampligen and/or CVP and their vaccine-specific serum IgG and IgA titers were measured on days 0 and 33. The vaccine was retained significantly longer in the nasal cavity of both mice and NHPs when it was delivered with CVP rather than PBS. Accum...Continue Reading

References

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Citations

Jan 28, 2017·Human Vaccines & Immunotherapeutics·Hui WuWenzhe Wu
Mar 10, 2017·Drug Development and Industrial Pharmacy·Jéssica Bassi da SilvaMarcos Luciano Bruschi
Jan 21, 2020·Microbiology and Immunology·Akira AinaiHideki Hasegawa
Aug 11, 2020·Expert Opinion on Drug Metabolism & Toxicology·Eita SasakiTakuo Mizukami
Aug 28, 2019·Current Immunology Reviews·Pamela A Kozlowski, Anna Aldovini

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