The Effect of Negative Pressure Wound Therapy with and without Instillation on Mature Biofilms In Vitro

Materials
Shamaila TahirK Vickery

Abstract

To investigate the effect of negative pressure wound therapy (NPWT) with and without instillation (NPWTi) on in vitro mature biofilm. Mature biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown under shear (130 rpm) on polycarbonate coupons in a CDC biofilm reactor for 3 days. Coupons containing biofilms were placed in a sterile petri dish and sealed using NPWT or NPWTi. Coupons were exposed to treatment for 24 h with NPWT alone or with instillation of: Povidone iodine solution (PVP-I) (10% w/v equivalent to 1% w/v available iodine, BETADINE®, Mundipharma, Singapore), surfactant based antimicrobial solution with polyhexamethylene biguanide (SBPHMB) (Prontosan®, B Braun Medical, Melsungen, Germany), Gentamicin 1 µg/mL (GM) (G1264 Sigma-Aldrich Pty Ltd., Castle Hill, Australia) Rifampicin 24 µg/mL (RF) (R3501 Sigma-Aldrich Pty Ltd., Castle Hill, Australia) and NaCl 0.9% (Baxter, Deerfield, IL, USA). Bacterial cell viability and biofilm architecture pre-and post-treatment were assessed using colony forming units (cfu), Live/Dead viability staining, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Significant reductions were obtained in S. aureus biofilm thickness (65%) and mass (...Continue Reading

References

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Citations

Nov 15, 2019·Chinese Medical Journal·Di WeiMin Zhang
Dec 21, 2019·International Wound Journal·Jason D HehrIan L Valerio
Aug 3, 2020·Plastic and Reconstructive Surgery·Ledibabari M NgaageYvonne M Rasko
Nov 19, 2019·Advances in Wound Care·Trevor HodsonIan L Valerio
May 8, 2020·Plastic and Reconstructive Surgery·Ledibabari M NgaageYvonne M Rasko
Apr 1, 2021·Plastic and Reconstructive Surgery. Global Open·Farid MeybodiMeagan Brennan

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Methods Mentioned

BETA
environmental stresses
confocal microscopy

Software Mentioned

ImageJ
Systat
Ulta
IMARIS
Sigma Plot

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