Abstract
To investigate the effect of negative pressure wound therapy (NPWT) with and without instillation (NPWTi) on in vitro mature biofilm. Mature biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown under shear (130 rpm) on polycarbonate coupons in a CDC biofilm reactor for 3 days. Coupons containing biofilms were placed in a sterile petri dish and sealed using NPWT or NPWTi. Coupons were exposed to treatment for 24 h with NPWT alone or with instillation of: Povidone iodine solution (PVP-I) (10% w/v equivalent to 1% w/v available iodine, BETADINE®, Mundipharma, Singapore), surfactant based antimicrobial solution with polyhexamethylene biguanide (SBPHMB) (Prontosan®, B Braun Medical, Melsungen, Germany), Gentamicin 1 µg/mL (GM) (G1264 Sigma-Aldrich Pty Ltd., Castle Hill, Australia) Rifampicin 24 µg/mL (RF) (R3501 Sigma-Aldrich Pty Ltd., Castle Hill, Australia) and NaCl 0.9% (Baxter, Deerfield, IL, USA). Bacterial cell viability and biofilm architecture pre-and post-treatment were assessed using colony forming units (cfu), Live/Dead viability staining, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Significant reductions were obtained in S. aureus biofilm thickness (65%) and mass (...Continue Reading
References
Feb 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chantal M MouësSteven E R Hovius
May 26, 2004·Annals of Plastic Surgery·Tonja WeedDavid B Drake
Mar 11, 2005·Microbiology·Darla M GoeresRodney M Donlan
Jan 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chandan K Sen
Jan 23, 2010·The Journal of Hospital Infection·R HadiT Charlton
Jul 7, 2010·FEMS Immunology and Medical Microbiology·Mette BurmølleThomas Bjarnsholt
Dec 1, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Quan D NgoAnand K Deva
Aug 10, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Erlangga YusufAndrej Trampuz
Nov 21, 2013·International Wound Journal·Priscilla L PhillipsGregory S Schultz
Apr 10, 2014·International Wound Journal·Pedro Miguel Ds ValenteKaren Vickery
Sep 10, 2014·Journal of Wound Care·J A SchwartzJ C Lantis
May 27, 2015·International Wound Journal·Subhas GuptaLuc Téot
Dec 1, 2012·The Journal of the American College of Clinical Wound Specialists·S G GossJ C Lantis
Sep 20, 2015·Diabetes/metabolism Research and Reviews·Benjamin A LipskyEdgar J G Peters
Nov 20, 2016·Expert Review of Anti-infective Therapy·Matthew MalonePaul Stoodley
Jun 15, 2017·The New England Journal of Medicine·David G ArmstrongSicco A Bus
Jul 4, 2017·EBioMedicine·M MaloneK Vickery
Citations
Jun 22, 2019·Surgical Innovation·Antonio BrillantinoMaurizio Castriconi
Nov 15, 2019·Chinese Medical Journal·Di WeiMin Zhang
Dec 21, 2019·International Wound Journal·Jason D HehrIan L Valerio
Jun 23, 2020·International Wound Journal·Paul J KimChristopher E Attinger
Jun 9, 2020·Experimental and Therapeutic Medicine·Tongtong LiPeifu Tang
Aug 3, 2020·Plastic and Reconstructive Surgery·Ledibabari M NgaageYvonne M Rasko
Jun 27, 2019·Materials·Karen Vickery
Nov 19, 2019·Advances in Wound Care·Trevor HodsonIan L Valerio
May 8, 2020·Plastic and Reconstructive Surgery·Ledibabari M NgaageYvonne M Rasko
Apr 1, 2021·Plastic and Reconstructive Surgery. Global Open·Farid MeybodiMeagan Brennan
Apr 17, 2021·Advances in Wound Care·Dean H MeshkinChristopher E Attinger
Jan 15, 2022·International Wound Journal·Lijiao WuKefeng Lin