PMID: 2484603Jul 1, 1989Paper

The effect of Ni(II) on DNA replication

Biological Trace Element Research
N T Christie, D M Tummolo

Abstract

The cellular regulation of DNA replication is governed in part by the availability of essential metal ions. A continuous supply of Mg(II) ions is necessary for the efficient and faithful replication of parental strands during S-phase as well as during the repair of DNA damage. A metal ion such as Ni(II) may interfere with the replication process by binding to sites on proteins at which essential ions normally bind. Binding at these sites by a toxic metal ion may produce inappropriate responses from the replication proteins and thus alter the normal balance in one or more of the microsteps comprising DNA synthesis. We have studied the effect of Ni(II) on DNA replication in a reconstituted in vitro system using a HeLa cell extract as a source of polymerase activity on a template of activated calf thymus DNA. Ni(II) has an initial stimulatory effect that is followed by an overall inhibition of the incorporation of DNA precursors. These results suggest that Ni(II), similar to Mg(II) may have more than one binding site, but that the binding of Ni(II) to replication proteins may significantly alter the timing of events in DNA synthesis.

References

Nov 1, 1979·Mutation Research·M MiyakiH Koyama
Nov 1, 1986·Somatic Cell and Molecular Genetics·G UrlaubJ Hamlin
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J J Li, T J Kelly
Jan 1, 1981·Toxicology·S K NiyogiD J Hoffman
Aug 1, 1981·Environmental Health Perspectives·F W Sunderman
Dec 1, 1982·Biological Trace Element Research·J D Heck, M Costa

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